LOCUS Exported 1004 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Mammalian expression of Cre recombinase fused to GFP. ACCESSION . VERSION . KEYWORDS pCAG-Cre:GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1004) AUTHORS Matsuda T, Cepko CL TITLE Controlled expression of transgenes introduced by in vivo electroporation. JOURNAL Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PUBMED 17209010 REFERENCE 2 (bases 1 to 1004) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1004 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(75..794) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(78..794) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(120..141) /label=EGFP-C /note="EGFP, forward primer" primer_bind 469..488 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 728..749 /label=EGFP-N /note="EGFP, reverse primer" regulatory 791..800 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga 61 ggagtgcggc cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc 121 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac 181 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc 241 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag 301 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag 361 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc 421 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg 481 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg 541 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc 601 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc 661 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg 721 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg 781 cccttgctca ccatggtggc gaccggtcca tcgccatctt ccagcaggcg caccattgcc 841 cctgtttcac tatccaggtt acggatatag ttcatgacaa tatttacatt ggtccagcca 901 ccagcttgca tgatctccgg tattgaaact ccagcgcggg ccatatctcg cgcggctccg 961 acacgggcac tgtgtccaga ccaggccagg tatctctgac caga //