LOCUS       Exported                 818 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized EGFP
            with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-link-yoEGFP-SpHis5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             32..745
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 acggatcggt gacggtgctg gtttaattaa catgtcaaaa ggcgaggaac tattcacagg
       61 tgtcgtacca atactggtag aattggatgg cgacgtaaac gggcataagt tttctgtgag
      121 tggtgagggt gaaggggacg caacttatgg caaacttaca cttaagttca tctgcaccac
      181 agggaagctg cctgtcccat ggccaactct agttacaact ctcacttacg gagtacaatg
      241 tttttctcga tacccagacc acatgaaaca acatgacttt ttcaaatctg ccatgcctga
      301 gggatatgtg caagagagaa ctattttctt taaagatgat ggtaattaca aaactcgtgc
      361 tgaagtgaag tttgaaggag atacacttgt taatagaatt gagttaaagg gtattgactt
      421 taaggaagat ggtaatatct taggtcacaa attggaatac aactacaatt cacataacgt
      481 ttacatcatg gcagataaac agaaaaacgg cataaaagtt aatttcaaaa ttagacataa
      541 tatcgaagat ggttccgttc aactcgctga tcattatcaa caaaatacac caatcggaga
      601 tggacctgtt ctattgccag acaaccatta cttatctaca cagagcgcct tgtcaaaaga
      661 tcctaatgaa aagagagatc acatggtttt gttagaattt gtcaccgctg cgggtataac
      721 gttgggcatg gatgaattat acaagtaagg cgcgccactt ctaaataagc gaatttctta
      781 tgatttatga tttttattat taaataagtt ataaaaaa
//
