LOCUS       Exported                6897 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMyNT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1348..1936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1837..1856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6682..6699
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6712..6732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      6829..6875
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagggg gcgtcaggcg ccgggggcgg tgtccggcgg cccccagagg aactgcgcca
       61 gttcctccgg atcggtgaag ccggagagat ccagcggggt ctcctcgaac acctcgaagt
      121 cgtgcaggaa ggtgaaggcg agcagttcgc gggcgaagtc ctcggtccgc ttccactgcg
      181 ccccgtcgag cagcgcggcc aggatctcgc ggtcgccccg gaaggcgttg agatgcagtt
      241 gcaccaggct gtagcgggag tctcccgcat agacgtcggt gaagtcgacg atcccggtga
      301 cctcggtcgc ggccaggtcc acgaagatgt tggtcccgtg caggtcgccg tggacgaacc
      361 ggggttcgcg gccggccagc agcgtgtcca cgtccggcag ccagtcctcc aggcggtcca
      421 gcagccgggg cgagaggtag ccccacccgc ggtggtcctc gacggtcgcc gcgcggcgtt
      481 cccgcagcag ttccgggaag acctcggaat ggggggtgag cacggtgttc ccggtcagcg
      541 gcaccctgtg cagccggccg agcacccggc cgagttcgcg ggccagggcg agcagcgcgt
      601 tccggtcggt cgtgccgtcc atcgcggacc gccaggtggt gccggtcatc cggctcatca
      661 ccaggtaggg ccacggccag gctccggtgc cgggccgcag ctcgccgcgg ccgaggaggc
      721 ggggcaccgg caccggggcg tccgccagga ccgcgtacgc ctccgactcc gacgcgaggc
      781 tctccggacc gcaccagtgc tcgccgaaca gcttgatcac cgggtcgggc tcgccgacca
      841 gtacggggtt ggtgctctcg ccgggcaccc gcagcaccgg cggcaccggc agcccgagct
      901 cctccagggc tcggcgggcc agcggctccc agaattcctg gtcgttccgc aggctcgcgt
      961 aggaatcatc cgaatcaata cggtcgagaa gtaacaggga ttcttgtgtc acnnnntcag
     1021 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct
     1081 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     1141 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     1201 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
     1261 gtatgagtca gcaacacctt cttcacgagg cagacctcac tagttccact gagcgtcaga
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1741 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     2101 ccgcacgcgt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca
     2161 ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct
     2221 cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt
     2281 tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag
     2341 tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat
     2401 acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag
     2461 cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc
     2521 caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca
     2581 aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg
     2641 gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca
     2701 gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca
     2761 tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca
     2821 ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat
     2881 ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg
     2941 cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc
     3001 gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct gccgacccgg
     3061 aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt
     3121 ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac
     3181 agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac
     3241 gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg
     3301 gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatgcatgg aggcattgct
     3361 atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa aaagcggcgc
     3421 gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc
     3481 gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga acgcatccgc
     3541 gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat
     3601 ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa
     3661 gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga
     3721 gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc
     3781 ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc
     3841 ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca
     3901 tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc
     3961 gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa
     4021 atctagataa agaagtgacg cggtctcaag cgtcgagcgt cgtcagcgtg tcgaggatgt
     4081 cgaagtcgta gccgtcggcg ctggcgatgt agacctgctg gtcgaattga ctgtcgcgca
     4141 tacacatcgg gccccggggc ccgtcgaacc cgacatcgtg cgcggatgcc atcaggtccg
     4201 gtatctcggg ggagtgggcc cgctggaaga tggcctcgag cgcaacgaga ccctcgtaac
     4261 aggattcggc catcgcgttg agcggtggcg cgtcggcgcc gtagcgggcg acgtagctgc
     4321 ccatcaggtc catcgcaccc gcggtggcca gtgaactgaa gtacgccgcg gcgacataga
     4381 ggttttcggt ggagccggcg ccgctggcca gcagcatgtt ctcctccatc agcgggctga
     4441 accgcgccat gcggtcgtgc ccgccggcgc gcgcgaactc gcggttgaac aacacggcgt
     4501 cctggccgac gagcagcatc aacacggcct gcgcccccga cgcgatggcc ttgcggacag
     4561 gtgcgcggaa atcgtcggtg ccgtacggga cgtagatctc ccgtctgagc tcgaggtcca
     4621 gatctcggca gtacgcgcgg gcggccgcgg cggaacggcg cggccagatg tagtcatcgc
     4681 cgaccaggca ccaggaccgg atgccgaagt ggtcgcgcag ccaggcgagc gcgggcgcga
     4741 tctggatctg cggtgtctcg cctgtgcaga acacgcccgg tgtgcgttca ccgccctcgt
     4801 acaacgaggt gtagacgtac gggatgcggt cgcggaccac cggggagatg cggttgcgca
     4861 cggccgagat gtgccagccg gtcacggcgt cgagaccgtg acctcgcaac cggtcggcga
     4921 cggtccgggc gacgtcgtcg ccgggcgctc cgccgtcgag cacctcgatg gtgaccttgc
     4981 ggccctgcag gccgcctcgg tcgttgacct ccttggccgc gagctcggcc acggcctcgc
     5041 acgaaggcgc gaagattccc gctggccctt gaagcggaat caccagcccg acgcggaact
     5101 caacctcgcc gtcctgcact ccagatcacc gtcgatcccg tgtagtctgc gcttcaaagg
     5161 tttctagcag aaataattca ttctgaacag accccgccgt cgacacgagg agacccaaca
     5221 tggccgccgg acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt ctgacccacc
     5281 tcgcggagtc ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc gaggactggc
     5341 gcgtgctcga cgagttggcc ggacggcgca ccgtgcccat gagcgatctc gcgcaggcca
     5401 cgctgatcac gggtccgact ctcaccagaa ccgtcgatcg ccttgtgtcg caagggatca
     5461 tctaccggac tgccgatctg catgaccgcc ggcgggtgct cgtggcgttg accccgcggg
     5521 ggcggacgct gcgcaaccgc ctggtggacg cggtagccga ggccgagtgt gcggcttttg
     5581 aatcgtgcgg gctggacgtc gaccagttgc gcgaactcgt cgacaccacc tcgaatttga
     5641 cttcgtaacc acccgcgccc ggccggcgtt cacccttgac ttttattttc atctggatat
     5701 atttcgggtg aatggaaagg ggtgaccatg ccgacctaca cattccgttg ttcccactgc
     5761 ggtcccttcg atctcacctg cgcgatctcc gagcgcgatg cggcggcgac ctgtccggag
     5821 tgccggacgc cggcgcgccg ggtcttcggt tcggtagggc tgacgacatt caccgcggga
     5881 catcaccgcg cattcgacgc ggcgtccgcg agcgccgaaa gtcccacggt ggtgaagtcg
     5941 attcccgcag gcgcggaccg cccgcgggcc ccgcgccgca atcccggtct accgagtctg
     6001 ccgaggtact agcgacatgg gtggcgtcgg gctcttctac gtgggtgcgg tgctcatcat
     6061 cgacgggctg atgctgctgg gccgcatcag cccacgaggc gcaacaccgc tgaacttctt
     6121 cgtcggcgga ctgcaggtgg tgacgcctac ggtgctgatc ctgcagtccg gcggagacgc
     6181 ggccgtgatc ttcgcggcct ccgggctcta cctgttcggc ttcacctacc tgtgggtggc
     6241 catcaacaac gtgaccgact gggacggaga aggtctcgga tggttctcgc tgttcgtcgc
     6301 gatcgccgca ctcggctact cgtggcacgc gttcaccgcc gaggccgacc cggcgttcgg
     6361 ggtgatctgg ctgctgtggg cagtgctgtg gttcatgctg ttcctgctgc tcggcctggg
     6421 gcacgacgca ctggggcccg ccgtcgggtt cgtcgcggtg gccgaaggcg tgatcaccgc
     6481 cgccgtgccg gccttcctga tcgtgtcggg caactgggaa accggcccgc tccccgccgc
     6541 ggtcatcgcc gtgatcggtt ttgccgcagt tgttctcgca taccccatcg ggcgccgtct
     6601 cgcagcgccg tcagtcacca accctccacc ggccgcgctc gcggccacca cccgataaga
     6661 gaaagggagt ccaccatgaa gcaccaccac caccaccacc cgtcggccgg cgagaacctg
     6721 tacttccagg gcgccatgga cgataaggta ccggatccga attcgatatc gctagcaagc
     6781 ttatcgatgt cgacgtagtt aactagcgta cgatcgactg ccaggcatca aataaaacga
     6841 aaggctcagt cgaaagactg ggcctttcgt tttatgccat catggccgcg gtgatca
//
