LOCUS       Exported               12691 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBbA5k-EPL63
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             9034..9051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9087..9158
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9174..9201
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(9229..9246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9363..9908)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9443..9462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      10022..10116
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10147..10941)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10258..10277)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10868..10887
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(11126..12208)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12170..12189
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12209..12286)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     12492..12511
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        12597..12627
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    12635..12651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgcttgtaa cgcatctgtg cttattgttc agccttctga agaagccccc acctgtaatg
       61 gttgatttat tcatgcaatc gtctggctct cgtaaatccc gtcgctggtt gatcagtttg
      121 ttgatcctgt tggccattgt tgccctttgc tggtggctct ggcctgcctc cactcccggc
      181 aaaggggccg agagcaagaa gcatggtgca ggtggcatgg cacggcctgg cttcggtgca
      241 tcggctgtcg ctgtcccggt gcgggtcgcc ccggcaaccg aaggcgattt ccctatctac
      301 tacaaggcgc ttggcacggt aacggcgatg aataccatca atgtgcgcag ccgtgtggcg
      361 ggagagctgg tcaagcttta tttccaggaa gggcagatgg tcaaggcggg tgacctgctg
      421 gcggaaatcg atccgcgcag ctatcaggtc gcattgcagc aggccgaagg tacgctggcc
      481 accaatcagg cgctgctcaa gaatgctcag ctcgacgtac agcgttaccg tgggcttttt
      541 gccgaggaca gcattgccaa gcagaccctc gacactgccg agtcgctggt ccatcagtac
      601 aaaggcacga tcaagaccaa tcaggctgcc gtggctgacg cgaagctgag cctggacttc
      661 acacgaattc gtgcgcctat tgcaggtcgt gtcggcctca aacagctgga tgtcggcaac
      721 ctggttgccg ccaacgacac cacggcgctg gtggtgatta cccagaccca gcccatttcg
      781 gtcgccttca cgctcccgga aaaggacctg tcgaaagtca tttcccgcta ccgcaccggc
      841 gacaagctgc ccgtcgaagc ctgggaccgt ggcgatacga aaatgcaggc ttccggtgta
      901 ctcgccagcc tcgataacca gatcgatgtg acgaccggca cactgaagtt caaggcccgt
      961 ttcgataacg gtgacgagat cctgttcccc aaccagttcg tcaatgtgcg tctgcgcgcc
     1021 gatacgttga aacaggtgac actggtgcca acggctgccg tgcagttcgg caccgacggg
     1081 acctttgtct atgtcctgga cggtgacaag aaggtcaaac tcaaactgtt gaagaccggc
     1141 ccgagcgacg agacatcgac ggtgatcacc gaaggtctgg cagccggaga acgggtcgtg
     1201 ctcgaaggca ccgaccgtct gcgcgacggc gctgaagtcg aggtcgtcaa cgacagcaag
     1261 gatgtaccgg cgggtccggt tcagaagctg cagggcaagc ctgacagcaa gacagacaag
     1321 tcggcagcac tcggcaaggt ggaaaaacca aacgtatgaa catgtcgcgc ctgttcatcc
     1381 tgcgtccggt tgccaccacg ctgagtatgc tggcaatcgt cctggcgggt ctgatcgcct
     1441 acaccttgct gccggtgtcg gcgctgcctc aggtcgacta tccgaccatt cgggtgatga
     1501 cgctgtatcc cggtgccagt ccgcaggtca tgaccagctc cgtcactgcg cctcttgagc
     1561 gtcaattcgg gcagatgccc ggtttgaccc agatggcctc gacgagctcg ggcggcgcgt
     1621 cggtcattac cctgcgtttc agcctcgaaa tcaatatgga tgtggctgag cagcaggtcc
     1681 aggccgcgat caacgccgcc accaaccttc tgcctaccga tcttccggca ccgccggtct
     1741 ataacaaggt caacccggca gacacgccgg ttctgaccct ggcgattacc tcgaaaacca
     1801 tgctgctgcc caagctcaat gagctggtcg atacacgcat ggcgcagaaa atctcgcaga
     1861 tcagcggtgt cggcatggtc agcattgccg gcggccagtg tcaggctgtg cggatcaagg
     1921 tcaatccgga agcgctggcc gccaacagcc tgaacctgtc tgatgtgcgg aacctgatcg
     1981 gcgcgtccaa cgtcaaccag cccaagggta acttcgacgg gccaacccgg gtttcgatgc
     2041 tggatgccaa cgatcagttg aagtctccag aggagtacgc caaccttatt ctggcctaca
     2101 aggatggcgc tcccctgcgt ctgaaggatg tggccgaaat cgtcgacggt gccgaaaacg
     2161 aacgcctggc ggcctgggcc aatcggagtc aggcggtgct gctgaacatt cagcgccagc
     2221 cgggcgccaa cgtgatcgaa gtggtcgacc ggatcaaggc attgctgccg tcaatcaccg
     2281 agaaccttcc ggccggcctc gacgtcgtgg tactgacgga ccgcactcag acgatccgcg
     2341 cgtcggtgac cgatgttcag cacgaactgt tgatcgcgat tatcctggtg gtgctggtaa
     2401 ccttcctgtt cctgcgtcgt ttcagcgcga cgatcattcc ctctatcgcc gtcccgctgt
     2461 cactggtagg caccttcggc gtcatgtacc tggcgggctt ctcggtcaac aacctcacgc
     2521 tgatggccat gaccatcgcg accggtttcg ttgttgatga cgccatcgtg atgctggaaa
     2581 acatctcccg gcatatcgag gagggcgaaa cacctctgca ggccgccctg aagggcgcga
     2641 aacagatcgg tttcaccctg atttccctga ccctgtcgct gattgcagtg cttataccgc
     2701 tgttgttcat ggctgatgtg gtcgggcgac tgttccgcga gttcgcgata accctggcag
     2761 tcgccatcct gatatcgctg gtggtatcgc tgaccctgac gcccatgatg tgcgcgcggt
     2821 tgctcaagcg cgagccaaag gaagaagagc agagccgttt ctatcgtgcc agcggtgcct
     2881 ggatcgactg gctgatcgat atctacgcgg gcgggttgcg ctgggtgctc aggcatcaac
     2941 cactgaccct gttggcggca ttggccactc tggcactgac tgtgctgctg tacatcgttg
     3001 tgcccaaggg cttttttccg gtgcaggaca ccggcgtgat ccagggtatt tcggaagcgc
     3061 cacagtcggt ctcctttgct gccatgagcc agcgtcagca ggcgctggct gacatcattc
     3121 tcaaggaccc tgccgtggtc agtctgtctt cctacattgg tgttgacggt gataacgcaa
     3181 cgctcaacag cgggcgtctg ctgatcaacc tcaagcccca ctcagagcgt gacctcaccg
     3241 catccgaggt gatccagcgc ctgcagccag aggtagacaa gctttcggac attcgtctgt
     3301 tcatgcagcc ggtgcaggac ctgaccattg aagaccgggt cagtcgtact cagtatcagt
     3361 tcagcatgtc atcgcccgac gccgagctgc tgacgctgtg gagcgaaagg ctggtcgagg
     3421 cgctcggcaa acggtccgag ctgacggatg tggccagcga cctgcaggac aagggcctac
     3481 aggtatatct gaacatcgat cgtgatgcgg ccagtcgggt aggggtcacc gttgcgaata
     3541 ttaccgatgc gctgtacgac gcattcggtc agcgacagat ttccaccatc tatacccagg
     3601 ccagccagta ccgcgtggtg ctgcaagccg cgtcgggcag tgaactcggc cctgccgcac
     3661 ttgagcagat ccacgtcaag accaccgatg gcgcacaggt aaaactgtcg agcctggcac
     3721 gcgtcgagca gcgtcaggcg cagttggcca ttgctcacct cgggcagttt cccgcagtga
     3781 tgatgtcgtt caaccttgcg cccggcgttg cattgggcaa ggccgtgcag gtcatcgaac
     3841 aggttgagca ggacatcggc atgccgattg gtgtgcagac ccagtttcag ggcgctgccg
     3901 aggcatttca ggcatcgttg tcgagcacgc tgctgctgat tctggcggct gtagtgacca
     3961 tgtacatcgt gctgggcgtg ctctacgaga gctatatcca cccgatcacc atcctctcga
     4021 cactgccgtc ggcagcagtg ggtgccttgc tggccttgct gatcagcggc aatgacctgg
     4081 ggatgatcgc gatcatcggt atcattttgc tgatcggcat cgtcaagaag aacgcgatca
     4141 tgatgatcga cttcgcgctc gacgcagagc gcaaccgggg tgttgcaccg gaaacggcga
     4201 tctacgaagc ggcgctgctg cgtttccggc cgattctcat gaccaccctg gcagcattgt
     4261 tcggcgccat tccgttgatg cttgccagcg gctccggcgc agagttgcgc cagccactgg
     4321 ggttggtgat ggtcggcggt ctgctcgtca gccaggtact gacgctgttt accacgccgg
     4381 tcatctatct gtatttcgac cggcttggcc gtcgctggag tcgcaggcct gccgacccgg
     4441 accgactgga acgggctgac gcatgaacct gtcagcgccg tttatccgtc gcccggttgc
     4501 gaccgtactg ctaagcctgg cgatcctgct gctgggcgcg gtcagcttcc gcttgttgcc
     4561 ggtcgcgccg ttgccgaata tggacttccc ggtgattgtg gtgtccgcca gtctggcagg
     4621 tgccagcccc gaggtaatgg cctccactgt ggccactccg ctggagcgtt cgctgggcag
     4681 tattgccggg gtcaatacca tgaccagcaa ttcaagccag ggcacgacac ggatcattct
     4741 gcagttcgat ctcaatcgcg acatcaatgg cgcggcgcgg gaagtgcagg cggcaatcaa
     4801 tgcttcacgc aacctgctgc ccagcggtat gcgcagcatg cctacctaca agaaggtcaa
     4861 cccgtcccag gcgccgatca tggtcctgtc tatgacgtcg acggtgctgg aaaaaggtca
     4921 gctttacgac ctggcatcca ccatcctgtc acagagcctg tcgcaggttt ccggggtcgg
     4981 cgaagtgcag atcggcggca gttcgctgcc agcggttcgt atcgagctgg agccacagat
     5041 gctctcccag tacggcgtat cgcttgacga cgtgcgcacc gcgattaccg gtgcgaacgt
     5101 gcggcggcca aagggttttg tggaggatga tcagcataac tggcaggttc aggccaacga
     5161 ccagctggaa accgcaaagg actatgcgcc gctgatcatt cgctacaagg atggggcgac
     5221 cttgcgcctc aaggacgtcg cgaaagtcag cgatgccgtg gaagatcgct acaacagcgg
     5281 gttttataac aatgaccggg cggtgttact ggtcgtcaac cgccaggcgg gtgccaacat
     5341 cattgaaacc gtggcgcaga tcaaggcgca attgccagcc ctgcgggccg tgctgcctgc
     5401 cagcgtgagc ctcaacgtcg cgatggatcg ctcgccggtg atcaaggcga cgctgcacga
     5461 ggcagagatg accctgctga ttgccgtggt tctggtggtg atggtggtat ttctgtttct
     5521 cggcagtttt cgtgcatcgc tgatccccac tctggcggtt ccggtatcgc tggtaggcac
     5581 ctttgcgatc atgcacctgc tcggtttttc cctgaacaat ctgtcgttga tggctttgat
     5641 cctcgccacc ggactggtgg tggacgatgc aatcgtggtg ctggaaaaca tttcccggca
     5701 cattcacaac ggtctcgacc ccatgaaagc ggcctttctg ggggccaaag aggtgggttt
     5761 caccttgctg tcaatgaacg tctcgctggt ggcggtgttc atttccattc tgttcatggg
     5821 tgggctggtc gaaagtctgt ttcgcgagtt ttccattacc ttgtcggtgt cgatcgtcgt
     5881 ctcgctgatc gtatccctga ccctgacgcc gatgttgtgc gcgcgctggc tcaaacccca
     5941 cgattcgggc aaggacaatg cctttcagcg ctggagcgag cgggtcaacg accgcatggt
     6001 cgccggctat gaccgctcgc tcggctgggt catgcggcat cgcaggctga cactgctcag
     6061 cctgctgatt accgttgtgg tgaatgtcgc gctgtatgtg gtggtgccca agacctttct
     6121 gccacagcag gacaccgggc aactgatggg ttttgtacgc ggtgacgacg gtctttcgtt
     6181 ctcggtcatg cagccgaaaa tggaaatctt tcgccgttcg attctggcag accctgcggt
     6241 ggagagcgtt gccggtttta tcggcggtag cggtggcacc aataacgcct tcatgatcgt
     6301 acgcctcaag ccgattgccg agcgcaagct gtctgccgaa aaagtggtcg agcgcctgcg
     6361 caagaacatg ccgcatgtgc ccggtggcag actattcttg gcgcctgatc aggacctgca
     6421 actggggggt ggtcgcgagc agaccagttc gcagtatcag tacattgtgc aaagtgcaga
     6481 cctgagcagc ctgcggctgt ggtatccgaa gatcgttgcg gcgctcaaat cgattccgga
     6541 gctgacggct atcgacgcca gggaagggcg cggggcgcag caagtgaccc tggtggtcaa
     6601 tcgtgatacg gccaaacggc tgggcatcga tatgaacatg gtcacggcgg tgctcaacaa
     6661 tgcctacagc cagcgtcagg tctcgaccat ctatgacagc ctcaaccagt acaaggtggt
     6721 gatggaggtc aatcccaaat acgctcagga cccggcgacg ctggagcagg ttcaggtgat
     6781 tactgccgac gggcagcgcg tgccgctgtc gagcattgct cattacgagc gcagtctggc
     6841 gaatgatcgg gtttcgcatg acggtcagtt cgctgccgaa aacatttcct ttgatctggc
     6901 cgaaggtgtc agcctcgaca aggcgacggt ggctatcgag cgcgcaattg ccgccatcgg
     6961 actgccgtcc gacatcattt ccaagatggc cgggaccgcc aacgccttcg cctcaaccca
     7021 gaaaagccag ccctggatga ttctcggtgc gttgctggcg gtgtatctgg tgctggggat
     7081 tctttatgaa agctacatcc atccattgac gatactctcg acgctgccat cggccggtgt
     7141 gggcgcattg ctgacgatct acgtgctggg cagcgaattc agcctgatct ccctgttggg
     7201 gctgttcctg ctgattggcg tggtgaagaa gaacgcaatc atgatgatcg acctggccct
     7261 gcaccttgag cgtgatcagg gaatgacgcc gcaagagtcg atccgcagcg cttgtctgca
     7321 acggctacgc cccattctga tgaccacaat ggccgccatt ctgggtgcat tgccgttgct
     7381 gctgagtacc gcggaaggcg ctgaaatgcg caaaccgctg ggcctgacca ttattggtgg
     7441 actgatcttc agccaggtcc tgactctcta caccaccccg gtggtttacc tttatctcga
     7501 ccgtctgcgc caccgttaca ataaatggcg tggcgtgcgt accgatgctg ctctggaaac
     7561 cccgctatga ccgaacacct ggcctttata tcccgcccga cagggctgat tcgtccactc
     7621 gcgctggggc tctgcgttgt gctgctcagc gcttgtgctg tcggtcctga ttaccagacg
     7681 ccgtcgatgg ccgcgcctgc gcagttcaag gccgccgaag gctggcgtgc agccacaccc
     7741 ggtgacgcga tggccaaagg tgcatggtgg gaggtgtaca gggatccgca actgaactcg
     7801 ctggtcgaac ggctcaacac ctccaaccag accgtcgccc agtacgatgc gcagtaccgg
     7861 caggcacaag cactggtgcg tagctccaga ggcgcatttc tgccgacact ggatctgacc
     7921 accggcaaga cccgctcggg gcagggcaca ggcacatcca ataccggcac gaataccggc
     7981 gcctcgggaa ttcgcaacag ttacaacgct caactgggcg tcagttggga agccgatgtg
     8041 tggggcaagt tgcgccgtgg gctggaggct gacaccgcca gtgcgcaggc aagtcttgcg
     8101 gacctggcag cgatgcgcct cagcctgcaa tcggagctgg tgcagaatta tctgcaattg
     8161 cgggtcattg acgaacagaa acgcctgctg gaatccactg tggacaccta tcagcgctct
     8221 ctgaccctca cccagaatca ataccgcgcc ggtatttcag gaagtgacgc tgtcgcccag
     8281 gcgcagacgc agttgaaaag tactcaggcc gacctgatcg atctggcctg gcagcgtgca
     8341 caattcgaga acgccattgc cgtgctggtg ggtatggcgc ccgctgactt caatctgccg
     8401 gccagcactt cgattccgca attgccgcag atcccgccag ggctgccgtc acagttgctt
     8461 gagcgccgac cggacattgc cgcggccgaa cgctcggtca tggccgccaa tgccaacatc
     8521 ggcgtcgcca aggccgctta ctacccagac ttcacgctga gcatgagcgg cggttacagc
     8581 agcagtacgt tcgccaactg gatcagcctg cccaaccgct tctggtcggt agggccgcaa
     8641 ctggcgttga cgttgttcga cggtggcagg cggacggcgg aagttgaccg taccgaggcc
     8701 gtgtacgacc agactgtcgc ccagtaccgg cagaccgtgc tgaatggttt tcaggaagtc
     8761 gaaaattacc tcgttcaact caaggtctac gagcaggagg ccgccgtgcg ccaggaagcg
     8821 ctggtatcgg ctcgcgactc gttgcgcctg accaacaacc agtacaaggc cgggctgatc
     8881 ggttatcttg acgtcgtcaa cgtacaggcc actgctttga gcaatgaacg cagtgtgctg
     8941 aacgttctgc aaagtcgctt gattgccagc gttcagttga ttgccgcatt gggtggcggc
     9001 tggaatgccg aaacaggttt gaccctcagg gagcatcatc accatcacca ctgaggatcc
     9061 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     9121 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     9181 cttcgggtgg gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga
     9241 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     9301 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     9361 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     9421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     9481 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     9541 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     9601 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9661 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     9721 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     9781 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     9841 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     9901 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     9961 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg
    10021 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
    10081 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag
    10141 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
    10201 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
    10261 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
    10321 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
    10381 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
    10441 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
    10501 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
    10561 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
    10621 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
    10681 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
    10741 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
    10801 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
    10861 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
    10921 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcatga
    10981 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
    11041 cccaacctta ccagagggcg ccccagctgg caattccgac gtcggtgcct aatgagtgag
    11101 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
    11221 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
    11281 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
    11341 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
    11401 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
    11461 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
    11521 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
    11581 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
    11641 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
    11701 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
    11761 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
    11821 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
    11881 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
    11941 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
    12001 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
    12061 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
    12121 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
    12181 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
    12241 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
    12301 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
    12361 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
    12421 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
    12481 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
    12541 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg tttaggcacc ccaggcttta
    12601 cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca atttcagaat
    12661 tcaaaagatc ttttaagaag gagatataca t
//
