LOCUS       Exported                 762 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJMK019
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 762)
  TITLE     Cohen Lab VIP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(16..37)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(250..306)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      318..327
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(334..357)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(361..393)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(397..414)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
       61 tttcgaaagt ttggactgca cgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      121 gccgatgggg gtgttctgct ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt
      181 gtggcggatc ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcct tgatatagac
      241 gttctcgagt gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt
      301 acgacgtgat gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg
      361 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
      421 tgatgcgcca ctaccggttc ggtcggcggc actgacatcg gcaccggcac tgggttctgg
      481 tgcctcggtg tcgcccagaa tagcccggga tctcaacagg ataaagccaa agccgacctt
      541 ggcagtcacg tccaacacca taaacagcag agtttcgatg cccaggccca ccacgccagc
      601 gccctcagtg cctatgatcc acaggatagg gtaagcggtc cacagcacca agaccagagc
      661 tgtcagggtg ttaaaggtag atgccacctc ggggccccgc tcctttgcag cagatcgcag
      721 ggatgtagcc agaaaataga gcaccactat catgcaaatt gt
//
