LOCUS       Exported                 892 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWzl_neo_DEST_flag_SOX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      17..26
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccggatcca gaattcgcca ccatggacta caaagacgat gacgacaagc aattgacaag
       61 tttgtacaaa aaagttggca tgtacaacat gatggagacg gagctgaagc cgccgggccc
      121 gcagcaaact tcggggggcg gcggcggcaa ctccaccgcg gcggcggccg gcggcaacca
      181 gaaaaacagc ccggaccgcg tcaagcggcc catgaatgcc ttcatggtgt ggtcccgcgg
      241 gcagcggcgc aagatggccc aggagaaccc caagatgcac aactcggaga tcagcaagcg
      301 cctgggcgcc gagtggaaac ttttgtcgga gacggagaag cggccgttca tcgacgaggc
      361 taagcggctg cgagcgctgc acatgaagga gcacccggat tataaatacc ggccccggcg
      421 gaaaaccaag acgctcatga agaaggataa gtacacgctg cccggcgggc tgctggcccc
      481 cggcggcaat agcatggcga gcggggtcgg ggtgggcgcc ggcctgggcg cgggcgtgaa
      541 ccagcgcatg gacagttacg cgcacatgaa cggctggagc aacggcagct acagcatgat
      601 gcaggaccag ctgggctacc cgcagcaccc gggcctcaat gcgcacggcg cagcgcagat
      661 gcagcccatg caccgctacg acgtgagcgc cctgcagtac aactccatga ccagctcgca
      721 gacctacatg aacggctcgc ccacctacag catgtcctac tcgcagcagg gcacccctgg
      781 catggctctt ggctccatgg gttcggtggt caagtccgag gccagctcca gcccccctgt
      841 ggttacctct tcctcccact ccagggcgcc ctgccaggcc ggggacctcc gg
//
