LOCUS Exported 843 bp ds-DNA linear SYN 21-AUG-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCFJ1200 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 843) TITLE Erik Jorgensen's plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 843) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..843 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3..110 /label=MCS /note="pBluescript multiple cloning site" primer_bind 27..43 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 27..43 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(77..93) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(78..94) /label=pBluescriptKS /note="For pBluescript vector" protein_bind complement(110..130) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(138..155) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(138..154) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(769..788) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 ttgagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat 61 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc aactttatta 121 tacatagttg ataattcact ggccgtcgtt ttacagaggg gagaaaatgc ggagagaaga 181 acccagcatc ttaataaaat tatataattt tgaataaaat caaattaaaa ataagattaa 241 ttgaaacaca acaatagaat gcagaaaatt actcataaga aataatttat tttgaaagaa 301 gttgtgtcga tttacgataa aaactacacg caaacgaggc tccgcgttta caccgtttgg 361 caatggagcg cgcttgctat tagattttag gccagctcaa cgaaagttta atttattttt 421 cttctttttt tccatttttt tgagtaaaat atcgaatttt ttgtattaat tctgtaaatt 481 ttataatttg caactatttt ggcgtattct gcttgttttt atcagcgaat ttttatgcat 541 ttcagtcaaa agtttgagca ggaaaaatga catttaggac tttgtaaaat tgtattctta 601 aatttgcttg aaaacctacg tgtacaattg attttattga atttttctta cgtttcccgt 661 tgtatcttat tgtatttaac tttcatcata aaataattta tttccaggta aaatatccga 721 cgctctcgtg gatccccggg attggctcag aaaaaaaagg taccgaccat gaccgagtac 781 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 841 gcc //