LOCUS       Exported                3243 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  empty backbone; high expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-MCSn1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Mergenthaler P, Kahl A, Kamitz A, van Laak V, Stohlmann K, Thomsen 
            S, Klawitter H, Przesdzing I, Neeb L, Freyer D, Priller J, Collins 
            TJ, Megow D, Dirnagl U, Andrews DW, Meisel A
  TITLE     Mitochondrial hexokinase II (HKII) and phosphoprotein enriched in 
            astrocytes (PEA15) form a molecular switch governing cellular fate 
            depending on the metabolic state.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1518-23. doi: 
            10.1073/pnas.1108225109. Epub 2012 Jan 10.
  PUBMED    22233811
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        314..591
                     /label=chicken beta-actin promoter
     primer_bind     1534..1556
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     misc_feature    1635..1691
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(1735..1754)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2068..2656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2557..2576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2742..2789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2781..2799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattaaca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
      361 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
      421 gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      481 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
      541 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc
      601 tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      661 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      721 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      781 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      841 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      901 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
      961 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1021 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1081 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1141 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1201 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     1261 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1321 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1381 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1441 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1501 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1561 gctctagaca attgtactaa ccttcttctc tttcctctcc tgacaggttg gtgtacagta
     1621 gcttccatgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
     1681 gcccgggatc caccggtcgc cagcggccgc tagctcgaca tgataagata cattgatgag
     1741 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1801 gctattgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1861 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1921 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtagatcc atttaaatgt
     1981 taattaacta gccatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2701 gccttttgct cacatgttct taattaaatt tttcaaaagt agttgacaat taatcatcgg
     2761 catagtatat cggcatagta taatacgact cactatagga gggccatcat ggccaagttg
     2821 accagtgctg tcccagtgct cacagccagg gatgtggctg gagctgttga gttctggact
     2881 gacaggttgg ggttctccag agattttgtg gaggatgact ttgcaggtgt ggtcagagat
     2941 gatgtcaccc tgttcatctc agcagtccag gaccaggtgg tgcctgacaa caccctggct
     3001 tgggtgtggg tgagaggact ggatgagctg tatgctgagt ggagtgaggt ggtctccacc
     3061 aacttcaggg atgccagtgg ccctgccatg acagagattg gagagcagcc ctgggggaga
     3121 gagtttgccc tgagagaccc agcaggcaac tgtgtgcact ttgtggcaga ggagcaggac
     3181 tgaggataag aattgagttt cagaaaaggg ggcctgagtg gccccttttt tcaacttaat
     3241 taa
//
