LOCUS Exported 10047 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLPCX-PECAMTL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10047) AUTHORS Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA TITLE Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1. JOURNAL Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 10.1016/j.cub.2013.04.049. PUBMED 23684974 REFERENCE 2 (bases 1 to 10047) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10047 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 414..589 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 652..851 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1052..1468 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1398..1420 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1436..1452 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 1566..2165 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(1566..1585) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2062..2082 /label=Puro-F /note="Puromycin resistance gene, forward primer" enhancer 2340..2643 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2644..2847 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2798..2818 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 5073..5780 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" CDS 5901..6620 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002; Kremers et al., 2006)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5946..5967) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6207..6226) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6554..6575 /label=EGFP-C /note="EGFP, forward primer" LTR 6786..7379 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(7482..7504) /label=pGEX 3' /note="pGEX vectors, reverse primer" protein_bind complement(7632..7665) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(7725..7758) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind 7778..7797 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(7878..7895) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8049..8637) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8129..8148) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8808..9668) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9431..9450 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9669..9773) /gene="bla" /label=AmpR promoter primer_bind 9841..9859 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctcgagctc aagcttagga tgcagccgag gtgggcccaa ggggccacga 2941 tgtggcttgg agtcctgctg acccttctgc tctgttcaag ccttgagggt caagaaaact 3001 ctttcacaat caacagtgtt gacatgaaga gcctgccgga ctggacggtg caaaatggga 3061 agaacctgac cctgcagtgc ttcgcggatg tcagcaccac ctctcacgtc aagcctcagc 3121 accagatgct gttctataag gatgacgtgc tgttttacaa catctcctcc atgaagagca 3181 cagagagtta ttttattcct gaagtccgga tctatgactc agggacatat aaatgtactg 3241 tgattgtgaa caacaaagag aaaaccactg cagagtacca gctgttggtg gaaggagtgc 3301 ccagtcccag ggtgacactg gacaagaaag aggccatcca aggtgggatc gtgagggtca 3361 actgttctgt cccagaggaa aaggccccaa tacacttcac aattgaaaaa cttgaactaa 3421 atgaaaaaat ggtcaagctg aaaagagaga agaactctcg agaccagaat tttgtgatac 3481 tggagttccc cgttgaggaa caggaccgcg ttttatcctt ccgatgtcaa gctaggatca 3541 tttctgggat ccatatgcag acctcagaat ctaccaagag tgaactggtc accgtgacgg 3601 aatccttctc tacacccaag ttccacatca gccccaccgg aatgatcatg gaaggagctc 3661 agctccacat taagtgcacc attcaagtga ctcacctggc ccaggagttt ccagaaatca 3721 taattcagaa ggacaaggcg attgtggccc acaacagaca tggcaacaag gctgtgtact 3781 cagtcatggc catggtggag cacagtggca actacacgtg caaagtggag tccagccgca 3841 tatccaaggt cagcagcatc gtggtcaaca taacagaact attttccaag cccgaactgg 3901 aatcttcctt cacacatctg gaccaaggtg aaagactgaa cctgtcctgc tccatcccag 3961 gagcacctcc agccaacttc accatccaga aggaagatac gattgtgtca cagactcaag 4021 atttcaccaa gatagcctca aagtcggaca gtgggacgta tatctgcact gcaggtattg 4081 acaaagtggt caagaaaagc aacacagtcc agatagtcgt atgtgaaatg ctctcccagc 4141 ccaggatttc ttatgatgcc cagtttgagg tcataaaagg acagaccatc gaagtccgtt 4201 gcgaatcgat cagtggaact ttgcctattt cttaccaact tttaaaaaca agtaaagttt 4261 tggagaatag taccaagaac tcaaatgatc ctgcggtatt caaagacaac cccactgaag 4321 acgtcgaata ccagtgtgtt gcagataatt gccattccca tgccaaaatg ttaagtgagg 4381 ttctgagggt gaaggtgata gccccggtgg atgaggtcca gatttctatc ctgtcaagta 4441 aggtggtgga gtctggagag gacattgtgc tgcaatgtgc tgtgaatgaa ggatctggtc 4501 ccatcaccta taagttttac agagaaaaag agggcaaacc cttctatcaa atgacctcaa 4561 atgccaccca ggcattttgg accaagcaga aggctagcaa ggaacaggag ggagagtatt 4621 actgcacagc cttcaacaga gccaaccacg cctccagtgt ccccagaagc aaaatactga 4681 cagtcagagt cattcttgcc ccatggaaga aaggacttat tgcagtggtt atcatcggag 4741 tgatcattgc tctcttgatc attgcggcca aatgttattt tctgaggaaa gccaaggcca 4801 agcagatgcc agtggaaatg tccaggccag cagtaccact tctgaactcc aacaacgaga 4861 aaatgtcaga tcccaatatg gaagctaaca gtcattacgg tcacaatgac gatgtcagaa 4921 accatgcaat gaaaccaata aatgataata aagagcctct gaactcagac gtgcagtaca 4981 cggaagttca agtgtcctca gctgagtctc acaaagatct aggaaagaag gacacagaga 5041 cagtgtacag tgaagtccgg aaagctgtcg agatggtgag caagggcgag gagaccacaa 5101 tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc 5161 acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca 5221 acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt 5281 tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc 5341 agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg 5401 tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag atacacctca 5461 agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg 5521 cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc 5581 tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga 5641 aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg 5701 acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac tccaccgacg 5761 gcatggacga gctgtacaag gggccaggtg gtgcagggcc aggtggtgca gggccaggtg 5821 gtgcagggcc aggtggtgca gggcccggtg gtgcaggtcc aggtggtgca ggtccaggtg 5881 gtgcaggtcc aggtggtgct atggtgagca agggcgagga gctgttcacc ggggtggtgc 5941 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 6001 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc 6061 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc 6121 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 6181 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 6241 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 6301 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 6361 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 6421 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 6481 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg 6541 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 6601 tggacgagct gtacaagtaa gcggccgcgg tccctgatgc cgtggaaagc agatactcta 6661 gaacggaagg ctcccttgat ggaacttagg aattcgttag gccattaagg cctgtcgaca 6721 agcggccgcc tcggccaaac atcgataaaa taaaagattt tatttagtct ccagaaaaag 6781 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc 6841 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat 6901 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 6961 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 7021 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 7081 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 7141 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 7201 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 7261 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 7321 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 7381 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 7441 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 7501 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 7561 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 7621 ggagtgtact gataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga 7681 gtttacgtcc agccaagcta gggccgcgat ccggaaccct taatataact tcgtataatg 7741 tatgctatac gaagttatca gtactggctt aactatgcgg catcagagca gattgtactg 7801 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 7861 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 7921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 7981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 8041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 8101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 8161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 8221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 8281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 8341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 8401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 8461 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 8521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 8581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 8641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 8701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 8761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 8821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 8881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 8941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 9121 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 9181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 9241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 9301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 9361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 9421 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 9481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 9541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 9601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 9661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 9721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 9781 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 9841 aataggcgta tcacgaggcc ctttcgtctt caagaattgc tagcaattgc tagcaattgc 9901 tagcaattca taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca 9961 aattcgcggg cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca 10021 aagccgcggc ccttccgttt ctttgct //