LOCUS Exported 1044 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Mammalian expression of ultrasensitive protein calcium sensor. ACCESSION . VERSION . KEYWORDS pGP-CMV-GCaMP6s SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1044) AUTHORS Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS TITLE Ultrasensitive fluorescent proteins for imaging neuronal activity. JOURNAL Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. PUBMED 23868258 REFERENCE 2 (bases 1 to 1044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1044 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 64..73 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 79..96 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 100..132 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 136..159 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" regulatory 166..175 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 187..243 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKTGHAVRAIGRLSS" primer_bind 456..477 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(583..604) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(844..863) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat 61 ctcgccacca tgggttctca tcatcatcat catcatggta tggctagcat gactggtgga 121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac catggtcgac 181 tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc 241 tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc 301 cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac 361 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc 421 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 481 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg 541 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 601 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc 661 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 721 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 781 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc 841 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 901 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 961 ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt taaagaggct 1021 ttctccctat ttgacaagga cggg //