LOCUS Exported 988 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.. ACCESSION . VERSION . KEYWORDS pBS-Hsp70-Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 988) TITLE FlyCRISPR JOURNAL Unpublished REFERENCE 2 (bases 1 to 988) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..988 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 148..169 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" CDS 220..285 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 292..312 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa 61 gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa 121 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt 181 gaatacaaga agagaactct gggggatctg atcgatatgg actataagga ccacgacgga 241 gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag 301 aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag catcggcctg 361 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc 421 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga 481 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga 541 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag 601 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag 661 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac 721 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc 781 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg 841 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg 901 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag 961 gccatcctgt ctgccagact gagcaaga //