LOCUS Exported 866 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pOSIP-KC (KanR, PhiC31) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 866) AUTHORS St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE TITLE One-step cloning and chromosomal integration of DNA. JOURNAL ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. PUBMED 24050148 REFERENCE 2 (bases 1 to 866) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..866 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 129..149 /label=BioBrick suffix /note="universal suffix for all parts" terminator 204..231 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 280..311 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" protein_bind 388..421 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" ORIGIN 1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 121 gctcacatta ctagtagcgg ccgctgcagg catgcctcga gatgcatggc gcctaaccta 181 aactgacagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttcg 241 aacaattgaa aaaacctcgc gccttacctg ttgagtaata gtcaaaagcc tccggtcgga 301 ggcttttgac tttctgctta ctgaatttcg gtggtgccgt taattaaccg gtgggccctc 361 atgataataa tggtttctta gacgtccgaa gttcctattc tctagaaagt ataggaactt 421 ccctaggtca gccaaacgtc tcttcaggcc actgactagc gataactttc cccacaacgg 481 aacaactctc attgcatggg atcattgggt actgtgggtt tagtggttgt aaaaacacct 541 gaccgctatc cctgatcagt ttcttgaagg taaactcatc acccccaagt ctggctatgc 601 agaaatcacc tggctcaaca gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa 661 agcttggctt ggagcctgtt ggtgcggtca tggaattacc ttcaacctca agccagaatg 721 cagaatcact ggcttttttg gttgtgctta cccatctctc cgcatcacct ttggtaaagg 781 ttctaagctt aggtgagaac atccctgcct gaacatgaga aaaaacaggg tactcatact 841 cacttctaag tgacggctgc atacta //