LOCUS       Exported                 874 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFT1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson 
            D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W
  TITLE     Conditional control of gene function by an invertible gene trap in 
            zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 
            Aug 20.
  PUBMED    22908272
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(221..268)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(359..392)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(439..472)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTACACAT). lox5171 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg
      121 ttcttctctt cttccgaccg cgaagagttt gtcgacctga aaaaaaaaag ggaagagaga
      181 gacacgtcag aaacacacac acactccgga ttagtctcga gaagttccta tactttctag
      241 agaataggaa cttcggaata ggaacttcac acacacacac acacacacac acgaagttcc
      301 tatactattt gaagaatagg aacttcggaa taggaacttc attaccctgt tatccctaat
      361 aacttcgtat aatgtatgct atacgaagtt atccaagcat caccatcgac cctctagtcc
      421 agatctcacc atcgacccat aacttcgtat aatgtgtact atacgaagtt atgggcccat
      481 ctggcctgtg tttcagacac cagggagtct ctgctcacgt ttcctgctat ttgcagcctc
      541 tctatcaaga ctaatacacc tcttcccgca tcggctgcct gtgagaggct tttcagcact
      601 gcaggattgc ttttcagccc caaaagagct aggcttgaca ctaacaattt tgagaatcag
      661 cttctactga agttaaatct gaggttttac aactttgagt agcgtgtact ggcattagat
      721 tgtctgtctt atagtttgat aattaaatac aaacagttct aaagcaggat aaaaccttgt
      781 atgcatttca tttaatgttt tttgagatta aaagcttaaa caagaatctc tagttttctt
      841 tcttgctttt acttttactt ccttaatact caag
//
