LOCUS Exported 9272 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 2nd generation lentiviral vector. ACCESSION . VERSION . KEYWORDS pHAGE PGK-GFP-IRES-LUC-W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9272) AUTHORS Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN TITLE Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. JOURNAL J Clin Invest. 2010 Jan . 120(1):379-89. PUBMED 20038801 REFERENCE 2 (bases 1 to 9272) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9272 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind complement(2261..2284) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 2611..2630 /label=mPGK-F /note="Mouse PGK promoter, forward primer" regulatory 2686..2695 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2692..3411 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2692..3408 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2737..2758) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2998..3017) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3345..3366 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3415..3982 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3432..3984 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3576..3593) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3803..3822 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3996..5648 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(4062..4080) /label=LucNrev /note="Firefly luciferase, reverse primer" misc_feature 5662..6250 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5715..5735) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6133..6144) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6325..6558 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6677..6695) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6763..6867 /gene="bla" /label=AmpR promoter CDS 6868..7728 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7086..7105) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7899..8487 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8388..8407 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8641..8658 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8750..8769 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8898..8919 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8934..8964 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8972..8988 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8977..8999 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8996..9012 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8996..9012 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9062..9081 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcatt 2221 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 2281 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 2341 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 2401 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 2461 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 2521 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg 2581 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 2641 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcggccgc catggtgagc 2701 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 2761 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 2821 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 2881 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 2941 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3001 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 3061 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3121 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 3181 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3241 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3301 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3361 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccctc 3421 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta 3481 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc 3541 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct 3601 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt 3661 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa 3721 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg 3781 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga 3841 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac 3901 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt 3961 cctttgaaaa acacgatgat aatatggcca cacatatgga agacgccaaa aacataaaga 4021 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg 4081 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg 4141 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac 4201 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct 4261 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt 4321 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt 4381 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa 4441 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca 4501 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg 4561 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg 4621 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca 4681 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa 4741 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg 4801 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc 4861 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt 4921 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca 4981 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta 5041 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt 5101 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg 5161 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca 5221 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag 5281 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg 5341 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg 5401 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc 5461 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg 5521 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa 5581 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg 5641 ccgtgtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 5701 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5761 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5821 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5881 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5941 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 6001 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 6061 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 6121 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 6181 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 6241 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 6601 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat 6661 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 6721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7081 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 8701 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag 8761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8821 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc 8881 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 8941 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 9001 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt 9061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 9121 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 9181 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 9241 atttgttaaa tatgtactac aaacttagta gt //