LOCUS Exported 6733 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION E. Coli/Gram-positive Bacterial Shuttle Vector. ACCESSION . VERSION . KEYWORDS pDL278 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6733) AUTHORS LeBlanc DJ, Lee LN, Abu-Al-Jaibat A TITLE Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. JOURNAL Plasmid. 1992 Sep;28(2):130-45. PUBMED 1409970 REFERENCE 2 (bases 1 to 6733) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6733 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(66..83) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(237..825) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(317..336) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 5155..5182 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 5274..5360 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 5653..5680 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 5772..5858 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(6024..6422) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGECRELFTTPRIGANQFLRRTVNAQTNPWQ NISIASAISSSRTRRISAVLADERRFSA" primer_bind 6317..6339 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6331..6348 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6332..6348 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 6349..6405 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(6418..6434) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6418..6434) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6431..6453) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6442..6458 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6466..6496) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6511..6532 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1201 agaagtggtc cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt 1261 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct 1321 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata 1381 ctgataagat aatatatctt ttatatagaa gatcgatttt cgttcgtgaa tacatgttat 1441 aataactata actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag 1501 gtttacactt actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa 1561 aattaactaa aataattatt atctagataa aaaatttaga agccaatgaa atctataaat 1621 aaactaaatt aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat 1681 agtgaggagg atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg 1741 gaaacattta aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg 1801 actaaaacca aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca 1861 aagtaaagaa atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag 1921 caacttacga tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca 1981 tcctcccaaa caagaattta tttatggaga atggttacaa gagctttatg aacaaggata 2041 cattcctcag aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa 2101 aaataaaaga atatacggaa attatgactt agaggaatta ctacctgata ttccattttc 2161 tgatgtgaga agagccatta tggattcgtc agaggaatta atagataatt atcaggatga 2221 tgaaaccaac tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat 2281 cataccaaaa gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga 2341 gagaattttg ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa 2401 tgtaaattta actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa 2461 aaatggtgga aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt 2521 cattctaatt ggtaatcaga ttttagaaaa caataaaccc ttgcataact ttctcgtcca 2581 tatcggaaac tactttttta ttcccttttt ttcgaccgag attattttgc gataaaatcc 2641 gattaagata ctgcctacta actcccaatt cttgagctaa ttctgatact gttttactca 2701 tcagctacct cacaaaataa ttctttctat cccaattcca aaaggcaaca atttcacgac 2761 cagtactttc tgcagattct tctgctccgg tctgaacaag attcccttct tcgacatcat 2821 caagagctaa ctccgccttg attttcttga acaactttcc aaaaccaatc tgcctttttc 2881 ggtgcaaacc attcgttaaa tcttctgtta tccgttcttt gaccatttca gagaataatg 2941 tcacatcttc tgtatctaca tcaaaaggct taacaggata tttagctgtt tcgataatag 3001 ctgacttcaa gtttttacgt ttatgagctt tgacggttcg aatatcaact acaggtgtat 3061 aatccaattt catcgcctta gcccatagtt tcgtccattc ttcttggcta atgtaatttg 3121 tattatttcc agtaaaatag ctagatttca caaataacaa tacatgcaaa tgagggtgat 3181 aattctcatg ctcagttgaa taagttacct cagtggctcg caaatagcca ataacatttt 3241 tatcgacttt cttgtatttc ataagacgat taaaagctcg ccctatttct gaaattgatt 3301 tatttaattt ttcaccacta atgttcttaa tcgtcaaggt caaaaagaga aagcgacctt 3361 ttggatagcg tttcattgct tcatttacca ctaattcagc ttgataagca tacttcattg 3421 accgtctcca gttacaaatt gggcagagct tatttttgca aaaataagac tgatagagtt 3481 ttttcgtgcc gtcttgttgt tcgataaact tcaacacttc ggcacattga tagacccgtt 3541 caaacgaacg atatcctaaa cgctctaaac gaccagcaag ctcaatattt tttaacttgc 3601 gttctcgcca ctttctatct ttgccattct tagaatagtc cttaaagact tgattttcag 3661 tacctgtcat gttataattt tcttgcaaaa aaattttttt gagtatataa aaaagttcct 3721 gcaattctcc taagtttatt attttaaatt ggacacttaa attataaact ttattttatt 3781 aaattgcaag ggcttttttg ttacctaaaa acccagcaat atcaagggtt tgaggtgctt 3841 taaaacaaga aaataaaaaa actcattatt tctatatgta tcaagataag aaaaaaccct 3901 tgctacgcaa ttgggttttt aagggctttt cagccctctt tttcgtttca cgaaaaattc 3961 acccctaaaa cctcaaattt tgactttcta aatccaaacg tggtataata attttaacgc 4021 tagaagttct aggaaacgaa tagcacaacg caaaaaaaac agtagctgga actactgtct 4081 ttcttttttg tcctcaaaaa gaactcaggt taatttttgt cgcgtttgtt tcgaacatac 4141 cgaatcaacc aatcagcact tacctctgca gcaacgccaa ccacaaaagg tatcaaaaca 4201 agttctagga aagtcttcac agcgctcacc tccaatctaa gttcgtaact tagcgaaaga 4261 acgttgcgac ctagctatta taccatgtcc gcttacttta ttccatggtc taattgacaa 4321 caagtaacca agggcggacg tcctttgtcc gtgtcggctc gaaacgctaa agcctttcgg 4381 ctcgtcacgt cctagcgtac tttgcccttg cttattgtca attagctttc acggcataaa 4441 tcgctcaaag gcctagccct ttttcaatca ctcgtttaac tacccttacg attggctgaa 4501 tagctcttaa ctctgatata aaatttttta aggctttatc tggcataaac tctttcagtt 4561 ttcctagcat tttgtcattt tgctgtctca aaactgagtt ttcacttcta agcgctctat 4621 tttcgttcct aagacgctct atgctctctc ccttactttt ttctttagct cgttccaact 4681 cgcttcaaac cgtttagagc gtctcagagg cactatccgc cttaattgct cattctctag 4741 ctctttttga tgaacaaggt ttctgcttcg gtatatcagc cctttaaatt cttcaaactg 4801 ctctttagtc aattccacat attctttacc gaacacgcct tttttagggg taatagccgc 4861 tagttctttc tcagacatga tttttaggtc agctatttgt gtttttagtt tttctaattt 4921 cagcccctcg cgctctaaac gctctatttt cgcttctaag ctcttcgaat aattttctaa 4981 ggtatttata tatccctcag ctttagatag ctctgagagc ctctcagagg tctcagaatc 5041 gccaggaccc aacgctgccc gactgctttc ctgatgcaaa aacgaggcta gtttaccgta 5101 tctgtggggg gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg 5161 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct 5221 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc 5281 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac 5341 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac 5401 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac 5461 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat 5521 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ctgctttcct gatgcaaaaa 5581 cgaggctagt ttaccgtatc tgtgggggga tggcttgtag atatgacgac aggaagagtt 5641 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca 5701 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc 5761 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 5821 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct 5881 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat 5941 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct 6001 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc 6061 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg 6121 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg 6181 aataattccc ggcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 6241 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 6301 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc 6361 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc 6421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6601 ccaacgcgcg gggagaggcg gtttgcgtat tggagcttgt tgtaactgaa aaaggaaaat 6661 tattgtgcca ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat 6721 gggaaacgta ttt //