LOCUS Exported 975 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules. ACCESSION . VERSION . KEYWORDS pCRY2PHR-mCherryN1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 975) AUTHORS Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL TITLE Rapid blue-light-mediated induction of protein interactions in living cells. JOURNAL Nat Methods. 2010 Oct 31. ():. PUBMED 21037589 REFERENCE 2 (bases 1 to 975) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..975 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(147..857) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(425..444) /label=mCherry-F /note="mCherry, forward primer" primer_bind 694..712 /label=mCherry-R /note="mCherry, reverse primer" regulatory 854..863 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 61 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct 121 gattatgatc tagagtcgcg gccgctttac ttgtacagct cgtccatgcc gccggtggag 181 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg 241 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg 301 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg 361 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc 421 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc 481 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg 541 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag 601 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac 661 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg 721 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg 781 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc 841 tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg ctgctgctcc gatcatgatc 901 tgtgcttcac gggttcttga aatagcttta gctagtagct cacgagctgt gtcgatgtct 961 acaatgggtt tcgca //