LOCUS Exported 2220 bp ds-DNA linear SYN 02-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL3540 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2220) AUTHORS Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR TITLE Targeted gene disruption in somatic zebrafish cells using engineered TALENs. JOURNAL Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PUBMED 21822241 REFERENCE 2 (bases 1 to 2220) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2220 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..69 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 76..96 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac 61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg 121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc 181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat 241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat 301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg 361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg 421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta 481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gaccccagac 541 caggtagtcg caatcgcgtc aaacggaggg ggaaagcaag ccctggaaac cgtgcaaagg 601 ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca 661 agcaatgggg gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt 721 caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgaataacaa tggagggaaa 781 caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca cggtttgacg 841 cctgcacaag tggtcgccat cgcctcgaat ggcggcggta agcaggcgct ggaaacagta 901 cagcgcctgc tgcctgtact gtgccaggat catggactga ccccagacca ggtagtcgca 961 atcgcgtcaa acggaggggg aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc 1021 ctttgtcaag accacggcct tacaccggag caagtcgtgg ccattgcaag caatgggggt 1081 ggcaaacagg ctcttgagac ggttcagaga cttctcccag ttctctgtca agcccacggg 1141 ctgactcccg atcaagttgt agcgattgcg aataacaatg gagggaaaca agcattggag 1201 actgtccaac ggctccttcc cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg 1261 gtcgccatcg ccagccatga tggcggtaag caggcgctgg aaacagtaca gcgcctgctg 1321 cctgtactgt gccaggatca tggactgacc ccagaccagg tagtcgcaat cgcgaacaat 1381 aatgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac 1441 cacggcctta caccggagca agtcgtggcc attgcatccc acgacggtgg caaacaggct 1501 cttgagacgg ttcagagact tctcccagtt ctctgtcaag cccacgggct gactcccgat 1561 caagttgtag cgattgcgtc gaacattgga gggaaacaag cattggagac tgtccaacgg 1621 ctccttcccg tgttgtgtca agcccacggt ttgacgcctg cacaagtggt cgccatcgcc 1681 agccatgatg gcggtaagca ggcgctggaa acagtacagc gcctgctgcc tgtactgtgc 1741 caggatcatg gactgacccc agaccaggta gtcgcaatcg cgtcgaacat tgggggaaag 1801 caagccctgg aaaccgtgca aaggttgttg ccggtccttt gtcaagacca cggccttaca 1861 ccggagcaag tcgtggccat tgcaaataat aacggtggca aacaggctct tgagacggtt 1921 cagagacttc tcccagttct ctgtcaagcc cacgggctga ctcccgatca agttgtagcg 1981 attgcgtcca acggtggagg gaaacaagca ttggagactg tccaacggct ccttcccgtg 2041 ttgtgtcaag cccacggttt gacgcctgca caagtggtcg ccatcgcctc gaatggcggc 2101 ggtaagcagg cgctggaaac agtacagcgc ctgctgcctg tactgtgcca ggatcatgga 2161 ctgacacccg aacaggtggt cgccattgct tcccacgacg gaggacggcc agccttggag //