LOCUS       Exported                 849 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAG_GPHN.FingR-eGFP-CCR5TC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman 
            ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB
  TITLE     Recombinant probes for visualizing endogenous synaptic proteins in 
            living neurons.
  JOURNAL   Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017.
  PUBMED    23791193
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             59..400
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             449..574
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          623..755
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     738..757
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(817..837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggacgagctg tactctaggg gagctggcgc tggagcgggt gcaggggctg gctctagatt
       61 ccagtgccgg atctgcatgc ggaacttcag cgaccggtcc aacctgagca ggcacatcag
      121 aacccacacc ggagaaaagc ccttcgcctg cgacatttgc ggccggaagt tcgccatcag
      181 cagcaacctg aacagccaca ccaagatcca cactggcagc cagaaacctt tccagtgcag
      241 aatttgtatg agaaacttta gcagaagcga caacctggcc agacacatcc ggacacatac
      301 tggtgaaaaa ccttttgcct gtgatatctg tggcagaaag tttgccacct ccggcaatct
      361 gacccggcac acaaagattc acctgcgggg cagccagcta tcgattgtcg acgctcctga
      421 acaacgtgaa ggtgcttctc aagtttctgt ttctgttact tttgaagatg ttgctgttct
      481 ttttactcgt gatgaatgga aaaaacttga tctttctcaa cgttctcttt atcgtgaagt
      541 tatgcttgaa aattattcta atcttgcttc tatggcttaa acgcgttgta caagtaaagc
      601 ggccccggac tcgaggccgc aggtaagtat caaggttaca agacaggttt aaggagacca
      661 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg
      721 tcttactgac atccactttg cctttctctc cacaggtgtc gacaatcaac ctctggatta
      781 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
      841 atacgctgc
//
