LOCUS Exported 5578 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pKT0176 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5578) AUTHORS Sheff MA, Thorn KS TITLE Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. JOURNAL Yeast 2004 Jun;21(8):661-70. PUBMED 15197731 REFERENCE 2 (bases 1 to 5578) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5578 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1425..1612 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(1480..1504) /label=pHybLex-R /note="ADH terminator, reverse primer" promoter 1687..2030 /label=TEF promoter /note="Ashbya gossypii TEF promoter" terminator 2849..3046 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(3153..3172) /label=T7 /note="T7 promoter, forward primer" promoter complement(3154..3172) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3259..3276) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3430..4018) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3510..3529) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4189..5049) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4812..4831 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5050..5154) /gene="bla" /label=AmpR promoter primer_bind 5222..5240 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5278..5300) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5400..5419 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 5500..5518 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 5500..5517 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 ttaattaaca tggtggcctc ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg 61 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 121 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc 181 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcgtacgt gaagcacccc 241 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 301 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc 361 acgctgatct acaaggtgaa gttccgcggc accaacttcc cccccgacgg ccccgtaatg 421 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg 481 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag 541 ttcaagacca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac 601 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc 661 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc 721 tccggcaccg cctcctccga ggacgtcatc aaagagttca tgcgcttcaa ggtgcgcatg 781 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 841 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg 901 gacatcctgt ccccccagtt ccagtacggc tccaaggcgt acgtgaagca ccccgccgac 961 atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 1021 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcacgctg 1081 atctacaagg tgaagttccg cggcaccaac ttcccccccg acggccccgt aatgcagaag 1141 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag 1201 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag 1261 accatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacaccaag 1321 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag 1381 ggccgccacc acctgttcct gtagggcgcg ccacttctaa ataagcgaat ttcttatgat 1441 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg 1501 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt 1561 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg 1621 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg 1681 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg 1741 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca 1801 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc 1861 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg 1921 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt 1981 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgacagtca 2041 acactaagac ctatagtgag agagcagaaa ctcatgcctc accagtagca cagcgattat 2101 ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc aattgacgtt gataccacta 2161 aggaattcct tgaattaatt gataaattag gtccttatgt atgcttaatc aagactcata 2221 ttgatataat caatgatttt tcctatgaat ccactattga accattatta gaactttcac 2281 gtaaacatca atttatgatt tttgaagata gaaaatttgc tgatattggt aataccgtaa 2341 agaaacaata tattggtgga gtttataaaa ttagtagttg ggcagatatt accaatgctc 2401 atggtgtcac tgggaatgga gtggttgaag gattaaaaca gggagctaaa gaaaccacca 2461 ccaaccaaga gccaagaggg ttattgatgt tagctgaatt atcatcagtg ggatcattag 2521 catatggaga atattctcaa aaaactgttg aaattgctaa atccgataag gaatttgtta 2581 ttggatttat tgcccaacgt gatatgggtg gccaagaaga aggatttgat tggcttatta 2641 tgacacctgg agttggatta gatgataaag gtgatggatt aggacaacaa tatagaactg 2701 ttgatgaagt tgttagcact ggaactgata ttatcattgt tggtagagga ttgtttggta 2761 aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa tgctggttgg aatgcttatt 2821 tgaaaaagac tggccaatta taatcagtac tgacaataaa aagattcttg ttttcaagaa 2881 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt 2941 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa 3001 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata 3061 ctaacgccgc catccagtgt cgaaaacgag ctcgaattca tcgatgatat cagatccact 3121 agtggcctat gcggccgcgg atctgccggt ctccctatag tgagtcgtat taatttcgat 3181 aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3601 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 3661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3841 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 3901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4201 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4261 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4321 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4381 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4441 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4501 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4561 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4621 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4681 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4741 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4801 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4861 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 4921 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 4981 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5041 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5101 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5161 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5221 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 5281 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 5341 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 5401 ggcatcagag cagattgtac tgagagtgca ccatatggac atattgtcgt tagaacgcgg 5461 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaac 5521 gcggccgcca gctgaagctt cgtacgctgc aggtcgacgg atcggtgacg gtgctggt //