LOCUS Exported 564 bp ds-DNA linear SYN 22-MAY-2021 DEFINITION mammalian expression of WT c-Jun. ACCESSION . VERSION . KEYWORDS Flag-JunWT-Myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 564) AUTHORS Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens A TITLE c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. JOURNAL Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 2011 Jan 2. PUBMED 21196933 REFERENCE 2 (bases 1 to 564) AUTHORS . TITLE Direct Submission JOURNAL Exported May 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..564 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 2..46 /label=CMVd3 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields strongly reduced expression relative to the full-length promoter" promoter 92..110 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 145..154 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 154..177 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" ORIGIN 1 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 61 tcagatccgc tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc 121 tggagctcca ccgcggtggc ggccgccacc atggattaca aggatgacga cgataaggcc 181 cgggcggatc ccccgggctg caggaattcg atgactgcaa agatggaaac gaccttctac 241 gacgatgccc tcaacgcctc gttcctccag tccgagagcg gtgcctacgg ctacagtaac 301 cctaagatcc taaaacagag catgaccttg aacctggccg acccggtggg cagtctgaag 361 ccgcacctcc gcgccaagaa ctcggacctt ctcacgtcgc ccgacgtcgg gctgctcaag 421 ctggcgtcgc cggagctgga gcgcctgatc atccagtcca gcaatgggca catcaccact 481 acaccgaccc ccacccagtt cttgtgcccc aagaacgtga ccgacgagca ggagggcttc 541 gccgagggct tcgtgcgcgc cctg //