LOCUS       Exported                 877 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    pInducer10-mir-RUP-PheS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     ncRNA           99..192
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     192..208
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     193..209
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             230..247
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             248..271
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      281..290
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 gctgtggcca agtactgcga cctccctagc aaactggggc acagatgatg cggccgcaag
       61 ccttgttaag tgctcgcttc ggcagcacat atactatgtt gaatgaggct tcagtacttt
      121 acagaatcgt tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc
      181 caacagaagg ctcgaggtcg acggtatcga taagcttgac gtccatatgc tggtaccccg
      241 tggaagtctg gaagttctct tccaggggcc cgaaggagac gccaccatgg gtctagatag
      301 ggataacagg gtaatggtac cggatcgatc ccccaacttc caggcccgcc atagtgattt
      361 gatttgccag cgcatcgcta tcaatcgccg ggttcaccca ttcgcgtaac cacagttcac
      421 tgaatttcat aatctattcc tgccttattt aaactgtttg aggaaacgca gatcgttttc
      481 gaagaatgaa cgcaggtcgg tgacgccgta acgcaacata gtcagacgct ccatccccat
      541 cccgaagccg aaaccagagt aaacttccgg gtcgatgcca acgttacgca acacgttcgg
      601 atgcaccatc ccgcagccca gcacttccag ccatttaccg tttttaccca tgacgtccac
      661 ttctgcagaa ggttcggtaa acgggaagta ggaaggacgg aagcgaatct gcaaatcttc
      721 ctcaaagaag ttacgcagga agtcgtgcag cgtgcctttc aggttggtaa agctgatgtt
      781 ggtatcaaca atcagacctt ccatctgatg gaacatcggc gtgtgagtct ggtcgtagtc
      841 gttacgataa acacggccag gcgcgatgat acgaatc
//
