LOCUS       Exported                 746 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSUPERIORpuro-shGAS5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Renganathan A, Kresoja-Rakic J, Echeverry N, Ziltener G, Vrugt B, 
            Opitz I, Stahel RA, Felley-Bosco E
  TITLE     GAS5 long non-coding RNA in malignant pleural mesothelioma.
  JOURNAL   Mol Cancer. 2014 May 23;13:119. doi: 10.1186/1476-4598-13-119.
  PUBMED    24885398
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     45..61
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     45..61
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(124..723)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(207..227)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     704..723
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gactactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat
       61 cccccgggct gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
      121 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
      181 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
      241 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
      301 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
      361 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
      421 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
      481 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
      541 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
      601 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
      661 gcgtacggcc ctggggacgt cgtcncgggt ggcgaggcgc accgtgggct tgtactcggt
      721 catggtaagc tagcttgggc tgcagg
//
