LOCUS       Exported                1006 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  mammalian expression of mutant c-Jun (four N-terminal residues 
            phosphorylated by JNK are mutated into Alanines).
ACCESSION   .
VERSION     .
KEYWORDS    Flag-Jun4A-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens
            A
  TITLE     c-Jun N-terminal phosphorylation antagonises recruitment of the 
            Mbd3/NuRD repressor complex.
  JOURNAL   Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 
            2011 Jan 2.
  PUBMED    21196933
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        55..73
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      108..117
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgat tacgccaagc tcgaaattaa
       61 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcc accatggatt
      121 acaaggatga cgacgataag gcccgggcgg atcccccggg ctgcaggaat tcgatgactg
      181 caaagatgga aacgaccttc tacgacgatg ccctcaacgc ctcgttcctc cagtccgaga
      241 gcggtgccta cggctacagt aaccctaaga tcctaaaaca gagcatgacc ttgaacctgg
      301 ccgacccggt gggcagtctg aagccgcacc tccgcgccaa gaactcggac cttctcacgg
      361 cgcccgacgt cgggctgctc aagctggcgg cgccggagct ggagcgcctg atcatccagt
      421 ccagcaatgg gcatatcacc actgcaccgg cccccaccca gttcttgtgc cccaagaacg
      481 tgaccgacga gcaggagggc ttcgccgagg gcttcgtgcg cgccctggct gaactgcata
      541 gccagaacac gcttcccagt gtcacctccg cggcacagcc ggtcagcggg gcgggcatgg
      601 tggctcccgc ggtggcctca gtagcaggcg ctggcggcgg tggtggctac agcgccagcc
      661 tgcacagtga gcctccggtc tacgccaacc tcagcaactt caacccgggt gcgctgagca
      721 gcggcggtgg ggcgccctcc tatggcgcgg ccgggctggc ctttccctcg cagccgcagc
      781 agcagcagca gccgcctcag ccgccgcacc acttgcccca acagatcccg gtgcagcacc
      841 cgcggctgca agccctgaag gaagagccgc agaccgtgcc ggagatgccg ggagagacgc
      901 cgcccctgtc ccctatcgac atggagtctc aggagcggat caaggcagag aggaagcgca
      961 tgaggaaccg cattgccgcc tccaagtgcc ggaaaaggaa gctgga
//
