LOCUS Exported 3020 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.. ACCESSION . VERSION . KEYWORDS pFAB3915 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3020) AUTHORS Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D TITLE Precise and reliable gene expression via standard transcription and translation initiation elements. JOURNAL Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PUBMED 23474465 REFERENCE 2 (bases 1 to 3020) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3020 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 97..191 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(222..1016) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(333..352) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 943..962 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter 1336..1365 /label=trc promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" CDS 1457..2134 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 2167..2238 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2254..2281 /label=T7Te terminator /note="phage T7 early transcription terminator" primer_bind complement(2324..2341) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2458..3003) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(2538..2557) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt 61 cagccccata cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg 121 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat 181 caacaggagt ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 241 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 301 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 361 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 421 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 481 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 541 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 601 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 661 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 721 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 781 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt 841 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 901 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 961 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 1021 aacgatcctc atcctgtctc ttgatcagat catgatcccc tgcgccatca gatccttggc 1081 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca 1141 gctggcaatt ccgacgtcga attcaaaaga tcttaagtaa gtaagagtat acgtatatcg 1201 gctaataacg tattaaggcg cttcggcgcc tttttttatg ggggtatttt catcccaatc 1261 cacacgtcca acgcacagca aacaccacgt cgaccctatc agctgcgtgc tttctatgag 1321 tcgttgctgc ataacttgac aattaatcat ccggctcgta taatgtgtgg agggcccaag 1381 ttcacttaaa aaggagatca acaatgaaag caattttcgt actgaaacat cttaatcatg 1441 cgggggagtg tttctaatgg cgagtagcga agacgttatc aaagagttca tgcgtttcaa 1501 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 1561 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 1621 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 1681 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 1741 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga 1801 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 1861 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 1921 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 1981 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 2041 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 2101 acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcg gttgtcgagt aaggatctcc 2161 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2221 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2281 gtttatagga tcctaactcg agcctaggga tatattccgc ttcctcgctc actgactcgc 2341 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc 2401 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt 2461 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg 2521 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc 2581 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca 2641 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg 2701 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2761 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta 2821 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct 2881 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg 2941 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct 3001 caagaagatc atcttattaa //