LOCUS Exported 992 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION TOP Hupki-puro2A-Hupki-wt plasmid . ACCESSION . VERSION . KEYWORDS pX61 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 992) AUTHORS Wei QX, Odell AF, van der Hoeven F, Hollstein M TITLE Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform. JOURNAL Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15. PUBMED 21445009 REFERENCE 2 (bases 1 to 992) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..992 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 10..43 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" primer_bind 949..969 /label=Puro-F /note="Puromycin resistance gene, forward primer" ORIGIN 1 cgcgggcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat 61 ctgaagcttc cgacttcttg gatacttgta actttgcgct ttcccaccct cgcataagtt 121 tcctgaaata atgactctga aactcaaaat atatttacaa acacctcggc tgtagctttg 181 gtttgttctc tgacttgctt ataacttaat atccctctta ttctaaccta agttctgcca 241 cgtggttggt tacctctgct cagcccccgg cttctgtcct ccatgttcct gggggaatcc 301 ctacactttc agaatttaat ttccctactg gatgtcccac cttcttttta ttctaccctt 361 tcctataagc cataggggtt tgtttgtttg tatgtttttt aattgacaag ttatgcatcc 421 atacagtaca caatctcttc tctctacaga tgggcaccga gtacaagccc acggtgcgcc 481 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 541 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 601 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 661 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 721 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 781 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 841 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 901 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 961 tctacgagcg gctcggcttc accgtcaccg cc //