LOCUS       Exported                 746 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  hypoxia-responsive enhanced green fluorescent protein (EGFP)-based 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    5HRE/GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Vordermark D, Shibata T, Brown JM
  TITLE     Green fluorescent protein is a suitable reporter of tumor hypoxia 
            despite an oxygen requirement for chromophore formation.
  JOURNAL   Neoplasia. 2001 Nov-Dec;3(6):527-34.
  PUBMED    11774035
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(6..35)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(216..237)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     565..584
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccattcagat cctcttctga gatgagtttt tgttctgcgg ccgcctacac attgatccta
       61 gcagaagcac aggctgcagg gtgacggtcc atcccgctct cctgggcaca agacatgggc
      121 agcgtgccat catcctgctc ctccacctcc ggcgggaagc cgtggctcag cttcttgtac
      181 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      241 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      301 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      361 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
      421 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
      481 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      541 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
      601 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      661 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
      721 ggcacgggca gcttgccggt ggtgca
//
