LOCUS Exported 889 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral shRNA vector (pGIPz based) for inducible knockdown. ACCESSION . VERSION . KEYWORDS pInducer10-mir-RUP-PheS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 889) AUTHORS Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ TITLE The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. JOURNAL Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. PUBMED 21307310 REFERENCE 2 (bases 1 to 889) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..889 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 134..736 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 633..653 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(811..831) /label=WPRE-R /note="WPRE, reverse primer" ORIGIN 1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 121 acacgataat accatggcca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 181 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 241 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 301 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 361 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat 421 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 481 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 541 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg 601 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 661 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 721 caagcccggt gcctgattaa ttaagcgcgt ctggaacaat caacctctgg attacaaaat 781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcatt //