LOCUS Exported 894 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pET21-Streptavidin-Glutamate_Tag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 894) AUTHORS Fairhead M, Krndija D, Lowe ED, Howarth M TITLE Plug-And-Play Pairing Via Defined Divalent Streptavidins. JOURNAL J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. PUBMED 24056174 REFERENCE 2 (bases 1 to 894) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..894 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 55..74 /label=T7 /note="T7 promoter, forward primer" promoter 55..73 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 74..98 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 113..135 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 572..589 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(642..660) /label=T7 Term /note="T7 terminator, reverse primer" terminator 656..703 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(827..846) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac 61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta 121 actttaagaa ggagatatac atatggctga agctggtatc accggcacct ggtacaacca 181 gctgggatcc accttcatcg ttaccgctgg tgctgacggt gctctgaccg gtacctacga 241 atccgctgtt ggtaacgctg aatctagata cgttctgacc ggtcgttacg actccgctcc 301 ggctaccgac ggttccggaa ccgctctggg ttggaccgtt gcttggaaaa acaactaccg 361 taacgctcac tccgctacca cctggtctgg ccagtacgtt ggtggtgctg aagctcgtat 421 caacacccag tggttgttga cctccggcac caccgaagct aacgcgtgga aatccaccct 481 ggttggtcac gacaccttca ccaaagttaa accgtccgct gcttccgaag aggaagagga 541 agagtaataa aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc 601 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc 661 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat 721 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 781 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 841 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgg //