LOCUS       Exported                 887 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET21-Streptavidin-Dead Aspartate Loop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Fairhead M, Krndija D, Lowe ED, Howarth M
  TITLE     Plug-And-Play Pairing Via Defined Divalent Streptavidins.
  JOURNAL   J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016.
  PUBMED    24056174
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             587..604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(657..675)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      671..718
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(842..861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggctga agctggtatc accggcacct ggtacgccca
      181 gctgggagac accttcatcg ttaccgctgg tgctgacggt gctctgaccg gtacctacga
      241 agccgctgtt ggtgatgacg atggtgacga tgacggtgat gacgatggag ctgaatctag
      301 atacgttctg accggtcgtt acgactccgc tccggctacc gacggttccg gaaccgctct
      361 gggttggacc gttgcttgga aaaacaacta ccgtaacgct cactccgcta ccacctggtc
      421 tggccagtac gttggtggtg ctgaagctcg tatcaacacc cagtggttgt tgacctccgg
      481 caccaccgaa gctaacgcgt ggaaatccac cctggttggt cacgacacct tcaccaaagt
      541 taaaccgtcc gctgcttcct aataaaagct tgcggccgca ctcgagcacc accaccacca
      601 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      661 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      721 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      841 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctt
//
