LOCUS       Exported                 922 bp ds-DNA     linear   SYN 07-JUL-2021
DEFINITION  Cas9 D10A+H840A.
ACCESSION   .
VERSION     .
KEYWORDS    Cas9m2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      65..74
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatcgaac
       61 ccttgccacc atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg
      121 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
      181 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
      241 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
      301 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
      361 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
      421 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
      481 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
      541 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
      601 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
      661 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
      721 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
      781 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
      841 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
      901 tctgctggcc cagatcggcg ac
//
