LOCUS Exported 948 bp ds-DNA linear SYN 07-JUL-2021 DEFINITION Cas9 D10A+H840A. ACCESSION . VERSION . KEYWORDS Cas9m2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 948) AUTHORS Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM TITLE CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. JOURNAL Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. PUBMED 23907171 REFERENCE 2 (bases 1 to 948) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..948 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 47..95 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 120..139 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" CDS complement(194..214) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca 61 acacccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat 121 tgtctccttc cgtgtttcag ttagcctccc ccgtttaaac tcattactaa ccggtaggga 181 tcgaaccctt tcacaccttc ctcttcttct tggggtcagc cctgctgtct ccaccgagct 241 gagagaggtc gattcttgtt tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt 301 ccaggacctc ctttgtagag gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga 361 aggctgcagg cgcgcccaag ttggtcagag taaacaagtg gataatgttt tctgcctgct 421 ccctgatggg cttatccctg tgcttattgt aagcagaaag caccttatcg aggttagcgt 481 cggcgaggat cactcttttg gagaattcgc ttatttgctc gatgatctca tcaaggtagt 541 gtttgtgttg ttccacgaac agctgcttct gctcattatc ttcgggagac cctttgagct 601 tttcatagtg gctggccaga tacaagaaat taacgtattt agagggcagt gccagctcgt 661 tacctttctg cagctcgccc gcactagcga gcattcgttt ccggccgttt tcaagctcaa 721 agagagagta cttgggaagc ttaatgatga ggtctttttt gacctcttta tatcctttcg 781 cctcgagaaa gtcgatgggg tttttttcga agcttgatcg ctccatgatt gtgatgccca 841 gcagttcctt gacgcttttg agttttttag acttcccttt ctccactttg gccacaacca 901 gtacactgta agcgactgta ggagaatcga atccgccgta tttcttgg //