LOCUS Exported 835 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.. ACCESSION . VERSION . KEYWORDS pdCas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 835) AUTHORS Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA TITLE Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. JOURNAL Nucleic Acids Res. 2013 Jun 12. PUBMED 23761437 REFERENCE 2 (bases 1 to 835) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..835 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 397..432 /label=DR /note="direct repeat for the Streptococcus pyogenes CRISPR/Cas system" repeat_region 463..498 /label=DR /note="direct repeat for the Streptococcus pyogenes CRISPR/Cas system" misc_feature complement(499..630) /label=crRNA leader /note="crRNA leader sequence for the Streptococcus pyogenes CRISPR/Cas system" ORIGIN 1 ccggccacga tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg 61 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg 121 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc 181 acgccatagt gactggcgat gctgtcggaa tggacgatca cactactctt cttttgccta 241 ttataacatg aaaacgcttt aacagaaaga ataggaaggt atccgactgc tggtattaac 301 cctctttctc aagttatcat cggcaatgtt gaatggagtc cattcaaaac agcatagctc 361 taaaacatgg aactcaacaa gtctcagtgt gctgaagttt tgggaccatt caaaacagca 421 tagctctaaa acgagacctt tgagcttccg agactggtct cagttttggg accattcaaa 481 acagcatagc tctaaaacct cgtagactat ttttgtctaa aaaatttcgt aatcgcacta 541 tttgtctcag ctagacttca gtcttgaaaa gcccctgtat tactgcattt attaagagta 601 ttataccata tttttagtta ttaagaaata atcttcatct aaaatatact tcagtcacct 661 cctagctgac tcaaatcaat gcgtgtttca taaagaccag tgatggattg atggataaga 721 gtggcatcta aaacttcttt tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa 781 tatttaaaag cagcgggagc tccaagattc gtcaacgtaa ataaatgaat aatat //