LOCUS Exported 186 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag. ACCESSION . VERSION . KEYWORDS pTD-NStrepHis SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 186) AUTHORS Dammeyer T, Timmis KN, Tinnefeld P TITLE Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein. JOURNAL Microb Cell Fact. 2013 May 20;12(1):49. PUBMED 23687945 REFERENCE 2 (bases 1 to 186) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..186 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 34..57 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 154..171 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 caattgttta aaggagatat acaaatggct agctggagcc acccgcagtt cgaaaaaggc 61 gccgagaccg cggtcccgaa ttcgagctcg gtacccgggg atccctcgag gtcgacctgc 121 agggggacca tggtctcagg cctgagagga tcgcatcacc atcaccatca ctaatttaaa 181 aagctt //