LOCUS       Exported                1434 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Genetically encoded H2O2 probe for ratiometric and fluorescence 
            lifetime imaging.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30-HyPer3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Bilan DS, Pase L, Joosen L, Gorokhovatsky AY, Ermakova YG, Gadella 
            TW, Grabher C, Schultz C, Lukyanov S, Belousov VV
  TITLE     HyPer-3: a genetically encoded H2O2 probe with improved performance 
            for ratiometric and fluorescence lifetime imaging.
  JOURNAL   ACS Chem Biol. 2012 Dec 20.
  PUBMED    23256573
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..1434
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="EMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLYQTFPKLEMYL
                     HEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPM
                     ADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQL
                     ADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELY
                     NVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTT
                     GKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLETL
                     RNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLA
                     EAIRARMDGHFDKVLKQAV"
     primer_bind     606..627
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(736..757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(997..1016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcacattgg tttgattccc
       61 acagttggac cgtacctgct accgcatatt atccctatgc tgtaccagac ctttccaaag
      121 ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc
      181 aaactcgatt gcgtgatcct cgcactggtg aaagagagcg aagcattcat tgaagtgccg
      241 ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa
      301 tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt
      361 ttgcgcgatc aggcaatgtc cgccggctac aacagcgaca acgtctatat catggccgac
      421 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacgtcga ggacggcagc
      481 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      541 cccgacaacc actacctgag cttccagtcc gtcctgagca aagaccccaa cgagaagcgc
      601 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      661 ctgtacaacg tggatggcgg tagcggtggc accggcagca agggcgagga gctgttcacc
      721 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      781 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
      841 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta cggcctgaag
      901 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      961 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1021 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcggc
     1081 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacggcac cggtttctgt
     1141 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga aactctgcgc
     1201 aacatggtgg cggcaggtag cgggatcact ttactgccag cgctggctgt gccgccggag
     1261 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
     1321 ggtctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
     1381 atccgcgcaa gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaa
//
