LOCUS Exported 1016 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION BIOFAB reporter plasmid for measuring T3 early termination efficiency. ACCESSION . VERSION . KEYWORDS pFAB804 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1016) AUTHORS Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D TITLE Precise and reliable gene expression via standard transcription and translation initiation elements. JOURNAL Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PUBMED 23474465 REFERENCE 2 (bases 1 to 1016) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1016 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 375..448 /label=PLtetO-1 promoter /note="modified phage lambda PL promoter with tet operator sites (Lutz and Bujard, 1997)" protein_bind 375..393 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 400..418 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" RBS 462..473 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" ORIGIN 1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag 61 atcatgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc 121 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccgacgtc tagggataac 181 agggtaatta cggccccaga attcaaaaga tcttaagtaa gtaagagtat acgtatatcg 241 gctaaaacgt attaaggcgc ttcggcgcct ttttttatgg gggtattttc atcccaatcc 301 acacgtccaa cgcacagcaa acaccacgtc gaccctatca gctgcgtgct ttctatgagt 361 cgttgctgca tatctcccta tcagtgatag agattgacat ccctatcagt gatagagata 421 ctgagcacat cagcaggacg cactgacctt tggaattcat taaagaggag aaaggtacca 481 tggcgagtag cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt 541 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta 601 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc 661 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg 721 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag 781 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca 841 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca 901 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa 961 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaacc //