LOCUS Exported 9267 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Lox-Stop-Lox TOPO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9267) AUTHORS Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, Jacks T, Tuveson DA TITLE Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. JOURNAL Genes Dev. 2001 Dec 15. 15(24):3243-8. PUBMED 11751630 REFERENCE 2 (bases 1 to 9267) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9267 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 239..257 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 239..256 /label=SP6 /note="SP6 promoter, forward primer" protein_bind complement(362..395) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 426..435 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 460..581 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(1006..1026) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 1156..1221 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" polyA_signal 1314..1435 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(1860..1880) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 2010..2075 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" polyA_signal 2168..2289 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(2714..2734) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 2864..2929 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" polyA_signal 3022..3143 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(3568..3588) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 3718..3783 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" promoter 4393..4892 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(4428..4451) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 4825..4844 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 4936..5535 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4936..4955) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5432..5452 /label=Puro-F /note="Puromycin resistance gene, forward primer" protein_bind complement(6076..6109) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(6154..6173) /label=T7 /note="T7 promoter, forward primer" promoter complement(6155..6173) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6180..6197) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6180..6196) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6189..6211) /label=M13/pUC Forward /note="In lacZ gene" CDS 6334..6636 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6515..6534 /label=ccdB-fwd /note="ccdB gene, forward primer" CDS 6985..7779 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7039..7058) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7649..7668 /label=Neo-F /note="Neomycin resistance gene, forward primer" regulatory 7980..7989 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7986..8360 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" rep_origin 8498..9086 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8987..9006 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9240..9257 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccaat ctcgagcctt 301 aattaaccac gcgtggcgcg ccttgagctc ggatccgggc cccccctcga tcgaggtcga 361 cataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct gcagacttac 421 tcaagccatg gtggctagct tgcggaattc cagacatgat aagatacatt gatgagtttg 481 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 541 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 601 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 661 acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt 721 attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat 781 agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt 841 atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc 901 tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg 961 actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt 1021 ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc 1081 ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt 1141 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac 1201 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta 1261 tgtcacacca cagaagtaag gttccttcac aaagatccct cgaccagaca tgataagata 1321 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 1381 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 1441 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 1501 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac 1561 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga 1621 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta 1681 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg 1741 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta 1801 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct 1861 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca 1921 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc 1981 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt 2041 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt 2101 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat ccctcgacca 2161 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 2221 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 2281 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 2341 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag 2401 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg 2461 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa 2521 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt 2581 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag 2641 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt 2701 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag 2761 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg 2821 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa 2881 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt 2941 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa 3001 agatccctcg accagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 3061 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 3121 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 3181 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg 3241 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat 3301 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct 3361 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt 3421 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta 3481 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa 3541 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg 3601 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt 3661 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta 3721 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt 3781 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta 3841 aggttccttc acaaagatcc ctcgatcgag aaaaaaaata taaaagagat ggaggaacgg 3901 gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa caagaaaagc 3961 atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca acgacaacaa 4021 cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca gaaataacat 4081 gagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg gcagcttctt 4141 tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa acaacggtgg 4201 cagcagatct aacggccgtt ctggtggtag atggatcgat ggcaaacatg tcccagctcc 4261 aagaaacgaa aaggccgaga tcgccatatt tggtgtcccc gaggatccct gtggaaaaaa 4321 aagggacagg ataagtatga catcatcaag gaaaccctgg actactgcgc ccttcgacct 4381 cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 4441 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 4501 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 4561 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 4621 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 4681 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 4741 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 4801 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 4861 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgatcgag 4921 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5521 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga 5581 ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct attaaacaat 5641 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca 5701 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta 5761 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag 5821 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 5881 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 5941 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 6001 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 6061 cagaagctgg tcgagataac ttcgtataat gtatgctata cgaagttatg tcgacagcgg 6121 ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca 6181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 6241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 6301 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga 6361 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga 6421 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 6481 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 6541 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 6601 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa 6661 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc 6721 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag 6781 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca 6841 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta 6901 aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa 6961 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 7021 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 7081 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 7141 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 7201 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 7261 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 7321 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 7381 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 7441 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 7501 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 7561 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 7621 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 7681 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 7741 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc 7801 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acaggtggca 7861 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7921 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac gtgaggaggg 7981 ccaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag 8041 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg 8101 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc 8161 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt 8221 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg 8281 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg 8341 tggccgagga gcaggactga cacgtgctaa aacttcattt ttaatttaaa aggatctagg 8401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 8461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 8521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 8581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 8641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 8701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 8761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 8821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 8881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 8941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 9001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 9061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 9121 gcttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 9181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 9241 gcgagtcagt gagcgaggaa gcggaag //