LOCUS Exported 11510 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLVPT-GDNF-tTR-KRAB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11510) AUTHORS Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P TITLE A versatile tool for conditional gene expression and knockdown. JOURNAL Nat Methods. 2006 Feb . 3(2):109-16. PUBMED 16432520 REFERENCE 2 (bases 1 to 11510) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11510 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 2..635 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 682..807 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1300..1533 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1717..1761 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2145 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2184..2683 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(2219..2242) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 2616..2635 /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 3381..3397 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3381..3397 /label=pBluescriptSK /note="For pBluescript vector" misc_feature 3433..3984 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3577..3594) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3804..3823 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3987..4604 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG" primer_bind complement(4060..4079) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" CDS 4608..4628 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4731..4925 /codon_start=1 /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /label=KRAB /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY QLTKPDVILRLEKGEEPWLV" primer_bind complement(5063..5079) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5064..5080) /label=pBluescriptKS /note="For pBluescript vector" misc_feature 5094..5682 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5147..5167) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5565..5576) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 5820..6090 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" LTR 6125..6305 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(6330..6348) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(6331..6348) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(6638..6657) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(6871..6890) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6990..7012 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7050..7068) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7136..7240 /gene="bla" /label=AmpR promoter CDS 7241..8101 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7459..7478) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8272..8860 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8761..8780 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9014..9031 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(9101..9121) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9106..9435 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9286..9421 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9348..9367 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 10569..10634 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 10764..10784 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 11209..11343 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11246..11265) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11300..11319 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga 1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa 2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact 2161 agcctcgacg atggtcgagt accgggtagg ggaggcgctt ttcccaaggc agtctggagc 2221 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 2281 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 2341 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 2401 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 2461 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 2521 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 2581 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 2641 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta gcgggatccc 2701 gtcgagaatt agcttcgcca ccatgaagtt atgggatgtc gtggctgtct gcctggtgct 2761 gctccacacc gcgtccgcct tcccgctgcc cgccggtaag aggcctcccg aggcgcccgc 2821 cgaagaccgc tccctcggcc gccgccgcgc gcccttcgcg ctgagcagtg actcaaatat 2881 gccagaggat tatcctgatc agttcgatga tgtcatggat tttattcaag ccaccattaa 2941 aagactgaaa aggtcaccag ataaacaaat ggcagtgctt cctagaagag agcggaatcg 3001 gcaggctgca gctgccaacc cagagaattc cagaggaaaa ggtcggagag gccagagggg 3061 caaaaaccgg ggttgtgtct taactgcaat acatttaaat gtcactgact tgggtctggg 3121 ctatgaaacc aaggaggaac tgatttttag gtactgcagc ggctcttgcg atgcagctga 3181 gacaacgtac gacaaaatat tgaaaaactt atccagaaat agaaggctgg tgagtgacaa 3241 agtagggcag gcatgttgca gacccatcgc ctttgatgat gacctgtcgt ttttagatga 3301 taacctggtt taccatattc taagaaagca ttccgctaaa aggtgtggat gtatctgagg 3361 atcaattcac gcgtggtacc tctagaacta gtggatcccc cgggctgcag gaattccgcc 3421 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 3481 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 3541 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 3601 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 3661 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 3721 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 3781 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 3841 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 3901 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 3961 cctttgaaaa acacgatgat aataccatgg ctagattaga taaaagtaaa gtgattaaca 4021 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc 4081 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc 4141 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag 4201 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa 4261 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa 4321 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat 4381 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc 4441 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac 4501 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat 4561 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcgcca aaaaagaaga 4621 gaaaggtcga cggcggtggt gctttgtctc ctcagcactc tgctgtcact caaggaagta 4681 tcatcaagaa caaggagggc atggatgcta agtcactaac tgcctggtcc cggacactgg 4741 tgaccttcaa ggatgtattt gtggacttca ccagggagga gtggaagctg ctggacactg 4801 ctcagcagat cgtgtacaga aatgtgatgc tggagaacta taagaacctg gtttccttgg 4861 gttatcagct tactaagcca gatgtgatcc tccggttgga gaagggagaa gagccctggc 4921 tggtggagag agaaattcac caagagaccc atcctgattc agagactgca tttgaaatca 4981 aatcatcagt ttaaggatct cgactagcta gtagctagct agctagtcga gctcaacttc 5041 gaattcgata tcaagcttat cgcgataccg tcgacctcga ctagtcatat gataatcaac 5101 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 5161 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 5221 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 5281 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 5341 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 5401 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 5461 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 5521 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 5581 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 5641 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcggtac gtatggccag 5701 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5761 aaggggggac tggaagggct aattcactcc caacgaagac aagatctcga gtttaccact 5821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 5881 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt 5941 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga 6001 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 6061 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcggatctgc tttttgcttg 6121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 6181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 6301 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg 6361 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat 6421 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga 6481 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg 6541 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg 6601 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat 6661 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg 6721 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg 6781 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat 6841 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag 6901 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 6961 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 7021 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 7081 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 7141 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 7201 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 7261 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 7321 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 7381 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 7441 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 7501 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 7561 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 7621 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 7681 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 7741 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 7801 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 7861 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 7921 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 7981 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 8041 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 8101 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 8161 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 8221 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 8281 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 8341 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 8401 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 8461 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 8521 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 8581 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 8641 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc 8701 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 8761 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 8821 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 8881 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 8941 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 9001 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 9061 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg 9121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 9181 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 9241 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 9301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 9361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 9421 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc 9481 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact 9541 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt 9601 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg 9661 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg 9721 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa 9781 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc 9841 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac 9901 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt 9961 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc 10021 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc 10081 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg 10141 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct 10201 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg 10261 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca 10321 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat 10381 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata 10441 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg 10501 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa 10561 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg 10621 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct 10681 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg 10741 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact 10801 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt 10861 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa 10921 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc 10981 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct 11041 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta 11101 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca 11161 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt 11221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 11281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 11341 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 11401 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt 11461 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag //