LOCUS       Exported               11583 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    pLVPRT-tTR-KRAB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb . 3(2):109-16.
  PUBMED    16432520
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             8..641
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    688..813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1539
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1723..1767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     intron          2383..2612
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2595..2614
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2717..2726
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2723..3439
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2723..3439
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2768..2789)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3029..3048)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3376..3397
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3535..4086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3679..3696)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3906..3925
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4089..4706
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(4162..4181)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4710..4730
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4833..5027
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    5169..5757
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5222..5242)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5640..5651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5895..6165
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6204..6384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6409..6427)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6410..6427)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6717..6736)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6950..6969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7069..7091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7129..7147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7215..7319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7320..8180
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7538..7557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8351..8939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8840..8859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9093..9110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9180..9200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9185..9514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9365..9500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9427..9446
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          10648..10713
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10843..10863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11288..11422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11325..11344)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11379..11398
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
       61 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
      121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
      181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
      241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
      301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
      361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
      421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
      481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      661 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      721 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      781 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      841 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      901 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      961 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1021 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1081 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1141 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1201 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1261 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1321 tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc tgacggtaca
     1381 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1441 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1501 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1561 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1621 acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1681 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1741 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1801 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1861 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1921 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1981 gacagagaca gattcgagat ctttacagtc aaatgagcca cgtcagggag cgatggcacc
     2041 cgcaggcggt atcaactgat gcaagtgttc aagcgaatct caactcgttt tttccggtga
     2101 ctcattcccg gccctggttg gcagcgctgc accctttaac ttaaacctcg gccggccgcc
     2161 cgccgggggc acagagtgtg cgccgggccg cgcggcaatt ggtccccgcg ccgacctccg
     2221 cccgcgagcg ccgccgcttc ccttccccgc cccgcgtccc tccccctcgg ccccgcgcgt
     2281 cgcctgtcct ccgagccagt cgctgacagc cgcggcgccg cgagcttctc ctctcctcac
     2341 gaagatccta gaattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     2401 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     2461 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     2521 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     2581 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcggac
     2641 tttcgatatc aagcttatcg atacccgtcg acctcgagat ccaggcctaa gcttacgcgt
     2701 cctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2761 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2821 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2881 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2941 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3001 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3061 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3421 catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct agctagctag
     3481 tcgagctcaa gcttcgaatt cccgggctgc aggaattccg cccccccccc cctaacgtta
     3541 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     3601 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     3661 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     3721 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3781 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     3841 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     3901 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3961 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4021 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     4081 ataataccat ggctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     4141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     4201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     4261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     4321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     4381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     4441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     4501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     4561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     4621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     4681 aaaaacaact taaatgtgaa agtgggtcgc caaaaaagaa gagaaaggtc gacggcggtg
     4741 gtgctttgtc tcctcagcac tctgctgtca ctcaaggaag tatcatcaag aacaaggagg
     4801 gcatggatgc taagtcacta actgcctggt cccggacact ggtgaccttc aaggatgtat
     4861 ttgtggactt caccagggag gagtggaagc tgctggacac tgctcagcag atcgtgtaca
     4921 gaaatgtgat gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc
     4981 cagatgtgat cctccggttg gagaagggag aagagccctg gctggtggag agagaaattc
     5041 accaagagac ccatcctgat tcagagactg catttgaaat caaatcatca gtttaaggat
     5101 ctcgactagc tagtagctag ctagctagtc gagctcaact tcgaattcga tgggactagt
     5161 catatgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5221 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5281 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5341 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5401 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5461 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5521 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5581 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5641 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5701 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5761 ggtacgtatg gccaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5821 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5881 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5941 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     6001 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6061 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgga
     6181 tcgatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6361 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     6421 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     6481 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     6541 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     6601 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     6661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     6721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     6781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     6841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     6901 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     6961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8461 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     8521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     8761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     9181 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9241 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9481 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     9541 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     9601 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     9661 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     9721 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     9781 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     9841 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     9901 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     9961 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    10021 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    10081 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    10141 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    10201 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    10261 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    10321 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    10381 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    10441 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    10501 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    10561 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    10621 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    10681 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    10741 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    10801 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    10861 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    10921 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    10981 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    11041 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    11101 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    11161 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    11221 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    11281 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11401 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    11461 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
    11521 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11581 act
//
