LOCUS Exported 9931 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLVUHshp53 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9931) AUTHORS Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P TITLE A versatile tool for conditional gene expression and knockdown. JOURNAL Nat Methods. 2006 Feb . 3(2):109-16. PUBMED 16432520 REFERENCE 2 (bases 1 to 9931) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9931 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2622..3833 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3670..3689 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 3854..3863 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3860..4579 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3860..4576 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3905..3926) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4166..4185) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4513..4534 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4620..5208 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4673..4693) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5091..5102) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5211..5227) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5212..5228) /label=pBluescriptKS /note="For pBluescript vector" promoter 5380..5594 /label=H1 promoter /note="human H1 RNA promoter" primer_bind 5515..5534 /label=H1 /note="Human H1 promoter, forward primer" protein_bind 5685..5718 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 5738..5918 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5942..5959) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5948..6172 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6218..6646 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6305..6324) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6515..6536 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6655..6675) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6660..6989 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6840..6975 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6902..6921 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7037..7084 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7103..7477 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7607..7728 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7644..7663) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7698..7717 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7777..7793) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7777..7793) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7790..7812) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7801..7817 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7825..7855) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7870..7891 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8008..8025) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8179..8767) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8259..8278) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8938..9798) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9561..9580 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9799..9903) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacaggatcc 3841 ccgggtaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 3901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 3961 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 4021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 4081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 4141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 4201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 4261 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 4321 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 4381 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 4441 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 4501 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 4561 ggacgagctg tacaagtaaa gcggccgcga ctctagaatt cgatatcaag cttatcgata 4621 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4681 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4741 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4801 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4861 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4921 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4981 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5041 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5101 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 5161 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc 5221 gacctcgagt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 5281 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca 5341 attcaccatg ctagtggatc ccccgggctg caggaattcg aacgctgacg tcatcaaccc 5401 gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct 5461 ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta 5521 tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt 5581 ctgtatgaga ccacagatcc ccgactccag tggtaatcta cttcaagaga gtagattacc 5641 actggagtct ttttggaaaa gcttatcgat accgtcgcat gggaataact tcgtatagca 5701 tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat 5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaaac ccgctgatca 5941 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 6001 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 6061 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 6121 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 6181 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 6241 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 6301 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 6361 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 6421 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 6481 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 6541 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 6601 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 6661 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 6721 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 6781 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 6841 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 6901 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 6961 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 7021 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 7081 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 7141 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 7201 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 7261 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 7321 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 7381 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 7441 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 7501 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 7561 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 7621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 7741 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 7801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9901 gcgcacattt ccccgaaaag tgccacctga c //