LOCUS Exported 954 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency. ACCESSION . VERSION . KEYWORDS pFAB822 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 954) AUTHORS Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D TITLE Precise and reliable gene expression via standard transcription and translation initiation elements. JOURNAL Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PUBMED 23474465 REFERENCE 2 (bases 1 to 954) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..954 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 352..425 /label=PLtetO-1 promoter /note="modified phage lambda PL promoter with tet operator sites (Lutz and Bujard, 1997)" protein_bind 352..370 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 377..395 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" RBS 439..450 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" ORIGIN 1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc atgatcccct gcgccatcag 61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag 121 ggcgccccag ctggcaattc cgacgtctag ggataacagg gtaattacgg ccccagaatt 181 caaaagatct taagtaagta agagtatacg tatatcggct aaaacgtatt aaggcgcttc 241 ggcgcctttt tttatggggg tattttcatc ccaatccaca cgtccaacgc acagcaaaca 301 ccacgtcgac cctatcagct gcgtgctttc tatgagtcgt tgctgcatat ctccctatca 361 gtgatagaga ttgacatccc tatcagtgat agagatactg agcacatcag caggacgcac 421 tgacctttgg aattcattaa agaggagaaa ggtaccatgg cgagtagcga agacgttatc 481 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 541 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 601 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 661 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 721 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 781 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 841 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 901 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaa //