LOCUS       Exported                 780 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  PesaR promoter, luxCDABE, and terminator in pET17b (AmpR).
ACCESSION   .
VERSION     .
KEYWORDS    pET-PesaRlux
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(174..278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     346..364
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      301 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      361 aggccctttc gtcttcacct cgaggcagat tgagtaaccg tgaatgtttg tacaaatgtt
      421 tcaaagatgt tactatgagt gtcccggcca gcatcacttt atattttgtg acgtctggcc
      481 ggacgttttc cctagtgttg gctgttttag cgacctggcc gtacaggtca ggtttttttt
      541 taccgctaaa caactgaagc cattgtaacc tctgaatgat tcattgtaag ttactcttaa
      601 gtatcatctt gcctgtacta tagtgcaggt taagtccacg ttaagtaaaa gaagcagcgg
      661 atcctctagt tgcggccgca aatggatggc aaatatgact aaaaaaattt cattcattat
      721 taacggccag gttgaaatct ttcccgaaag tgatgattta gtgcaatcca ttaattttgg
//
