LOCUS Exported 850 bp ds-DNA linear SYN 06-JUL-2021 DEFINITION esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR). ACCESSION . VERSION . KEYWORDS pAC-EsaR-I70V-EsaI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 850) AUTHORS Shong J, Collins CH TITLE Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. JOURNAL ACS Synth Biol. 2013 Jul 23. PUBMED 23879176 REFERENCE 2 (bases 1 to 850) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..850 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 110..131 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 146..176 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 184..200 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 189..211 /label=M13/pUC Reverse /note="In lacZ gene" RBS 218..229 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" ORIGIN 1 tttctagacc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 181 gaattgtgag cggataacaa tttcacacag gaaacataaa gaggagaaag gtacccatga 241 tgttctcttt cttccttgaa aaccaaacaa taacggatac gcttcagact tacatacaga 301 gaaagttatc tccgctgggt agtccggatt acgcttacac tgttgtgagc aaaaaaaatc 361 cttcaaatgt tctgattatt tccagttatc ctgacgaatg gattaggtta taccgcgcta 421 acaactttca gctgaccgat ccggttgttc tcacggcctt taaacgcacc tcgccgtttg 481 cctgggatga gaatattacg ctgatgtccg acctgcggtt caccaaaatt ttctctttat 541 ccaagcaata caacatcgtt aacggcttta cctatgtcct gcatgaccac atgaacaacc 601 ttgctctgtt gtccgtgatc attaaaggca acgatcagac tgcgctggag caacgccttg 661 ctgccgaaca gggcacgatg cagatgctgc tgattgattt taacgagcag atgtaccgcc 721 tggcaggcac cgaaggtgaa cgagccccgg cgttaaatca gagcgcggac aaaacgatat 781 tttcctcgcg tgaaaatgag gtgttgtact gggcgagtat gggcaaaacc tatgctgaga 841 ttgccgctat //