LOCUS Exported 1011 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION FRET sensor for beta2-adrenergic receptor . ACCESSION . VERSION . KEYWORDS pCDNA_B2AR-s-pep SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1011) AUTHORS Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, Sivaramakrishnan S TITLE Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells. JOURNAL J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 10.1074/jbc.M113.464065. Epub 2013 Apr 29. PUBMED 23629648 REFERENCE 2 (bases 1 to 1011) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1011 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 89..802 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 89..802 /codon_start=1 /product="enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(131..152) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(392..411) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 739..760 /label=EGFP-C /note="EGFP, forward primer" CDS 827..847 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" ORIGIN 1 cattgattca caagggagga attgtagtac aaatgactca ctgctgtcta gaggaagcgg 61 aggaagcgga ggaagcggag gaagcggagt gagcaagggc gaggagctgt tcaccggggt 121 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 181 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 241 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt 301 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 361 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 421 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 481 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 541 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 601 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 661 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc 721 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 781 cggcatggac gagctgtaca aggaattcgg aagcggagga agcggagaaa acctgtattt 841 tcagggcgga agcggagaag aggaagagaa aaaaaaacag caggaagagg aagcagaaag 901 gctgaggcgt attcaagaag aaatggaaaa ggaaagaaaa agacgtgaag aagatgaaca 961 acgtcgaaga aaggaggagg aggaaaggcg gatgaaactt gagatggaag c //