LOCUS       Exported                1099 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  An AAV vector that expresses engineered halorhodopsin 3.0 (fused to 
            iRFP) under the CaM Kinase IIa promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(101..1048)
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attctcatta cacctcgttc tcgtagcaga acttgtacag ctcttccatc acgccgatct
      121 gccaggccag aacctgggcg actagctcgc aggcttggcg gccatcgaga tcgacgtagt
      181 acggcgttcg gtgatggcaa acgatcaatc cccacagtcg ctcgccgcgc aaaatcgaga
      241 tcgacatcgt gccgtgcatg cctatgttgc gcatgaattc cagatggacg ggcgagacgc
      301 tgcgcaggat ggcgaagcta agatcaatcg gccgcccggt gaccggattg aggtctgggg
      361 tgaccggcac cggccgataa ttgatatcgg gaatgatccg taccgggttg atggtataga
      421 gccgacgggc ctgcgccggc acggttgagg caggatagtg caggcctagt tttgactcga
      481 cctcggcgca ccgatcctct gcgatcactt cgccgctgaa gtcggaggcg aagcgataga
      541 tcatcacccg atcgaagccg gtaatcttcc gcacctcttg cgccgcggcg gcgcaggcgc
      601 tttccaaggt ttcggcggcc tgcaggcggc ggatggcgct gttggtgcgg cggaagaacg
      661 cctgcggctc ggcgacgtcc cgctggggag gctcgagctc gaggaagatg agctgatcat
      721 ggcgatgcca ggagccgatg aagcctgcgt cctttcgcat cgtgaagccg acagtgatcg
      781 gtgctccgac ggccgccccg ggctcggcca aggcgatcgt cagacggttg tgcgtctccg
      841 agtcgaagac atcggccgca gagcggccga tcagggcgcc gatcgccagt ccggtgagtt
      901 cgggaaggtt gtcgctgccg gcaacgatcg tcatgtcggc ggcgagggcg agcagcagtc
      961 catgcggttg gatggcaccg gggatatgga tcggctcatc gtcgcaggtc aagaggtcag
     1021 gctgcctggc gacggatcct tcagccatac tagtcaccac gttgatgtcg atctggtcca
     1081 gggggatgta ctcgccctc
//
