LOCUS       Exported                1068 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) under the CaM Kinase IIa promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(71..1021)
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcgtcgac tcactcttcc atcacgccga tctgccaggc cagaacctgg gcgactagct
      121 cgcaggcttg gcggccatcg agatcgacgt agtacggcgt tcggtgatgg caaacgatca
      181 atccccacag tcgctcgccg cgcaaaatcg agatcgacat cgtgccgtgc atgcctatgt
      241 tgcgcatgaa ttccagatgg acgggcgaga cgctgcgcag gatggcgaag ctaagatcaa
      301 tcggccgccc ggtgaccgga ttgaggtctg gggtgaccgg caccggccga taattgatat
      361 cgggaatgat ccgtaccggg ttgatggtat agagccgacg ggcctgcgcc ggcacggttg
      421 aggcaggata gtgcaggcct agttttgact cgacctcggc gcaccgatcc tctgcgatca
      481 cttcgccgct gaagtcggag gcgaagcgat agatcatcac ccgatcgaag ccggtaatct
      541 tccgcacctc ttgcgccgcg gcggcgcagg cgctttccaa ggtttcggcg gcctgcaggc
      601 ggcggatggc gctgttggtg cggcggaaga acgcctgcgg ctcggcgacg tcccgctggg
      661 gaggctcgag ctcgaggaag atgagctgat catggcgatg ccaggagccg atgaagcctg
      721 cgtcctttcg catcgtgaag ccgacagtga tcggtgctcc gacggccgcc ccgggctcgg
      781 ccaaggcgat cgtcagacgg ttgtgcgtct ccgagtcgaa gacatcggcc gcagagcggc
      841 cgatcagggc gccgatcgcc agtccggtga gttcgggaag gttgtcgctg ccggcaacga
      901 tcgtcatgtc ggcggcgagg gcgagcagca gtccatgcgg ttggatggca ccggggatat
      961 ggatcggctc atcgtcgcag gtcaagaggt caggctgcct ggcgacggat ccttcagcca
     1021 tactagtggc ggccgctggc acggctccgg cctcggcttc gtcttcga
//
