LOCUS       Exported                 800 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression.
ACCESSION   .
VERSION     .
KEYWORDS    pAC149-pCR8-dCas9VP160
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(139..522)
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(556..576)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattctt
      121 aattaatcaa tcgatataca acatatccaa atcgaagtca tcgagcgcat cggagcccag
      181 catgtccagg tcaaagtcgt ccagggcatc actccccagc atatccaagt caaaatcatc
      241 caaggcatcg gaccccagca tgtccagatc gaaatcgtcc agggcgtcgc tgccgagcat
      301 gtccaggtcg aaatcatcaa gggcatcgct tcccaacata tcaaggtcga aatcatccag
      361 tgcgtctgac cctaacatat cgagatcgaa atcgtccaga gcatcggagc cgagcatgtc
      421 cagatcaaag tcatccaatg cgtcgcttcc caacatatcc aggtcaaagt catcgagggc
      481 atcagaaccc agcatgtcga gatcgaaatc gtccagcgcg tcggcgcgcc cggatccggc
      541 cggcccgctg gcctccacct ttctcttctt cttggggctg tcgcctccca gctgagacag
      601 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      661 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      721 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      781 gggcttatcc cggtgcttgt
//
