LOCUS       Exported                 775 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  sgRNA expression on PiggyBac vector with Neo-selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    pAC103-PBNeo-U6-sgRNA-Expression
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      301 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
      361 atggcacgcg cctggatccc cgggtaccga gttgggagct cacggggaca gccccccccc
      421 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
      481 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
      541 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
      601 gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag
      661 aatgacagtc tagtgggagc tcacggggac agcccccccc caaagccccc agggatgtaa
      721 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccgg
//
