LOCUS Exported 2951 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Contains 8xLexA-binding elements. ACCESSION . VERSION . KEYWORDS 8xLexA-binding elements/pMD20 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2951) AUTHORS Fujita T, Fujii H TITLE Direct identification of insulator components by insertional chromatin immunoprecipitation. JOURNAL PLoS One. 2011;6(10):e26109. doi: 10.1371/journal.pone.0026109. Epub 2011 Oct 17. PUBMED 22043306 REFERENCE 2 (bases 1 to 2951) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2951 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 23..40 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 23..40 /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(341..358) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(341..357) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(350..372) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(565..584) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 684..706 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(744..762) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 830..934 /gene="bla" /label=AmpR promoter CDS 935..1795 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1153..1172) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1966..2554 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2455..2474 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2708..2725 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2842..2863 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2878..2908 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2916..2932 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2921..2943 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2940..5 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2940..5 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 atgaccatga ttacgccaag ctatttaggt gacactatag gggaaagctt gcatgcctgc 61 aggtcgactc tagaggatct actagtcata tggattaatc tagattctct atcgataggt 121 acctcgactg ctgtatataa aaccagtggt tatatgtaca gtactgctgt atataaaacc 181 agtggttata tgtacagtac gcctcgactg ctgtatataa aaccagtggt tatatgtaca 241 gtactgctgt atataaaacc agtggttata tgtacagtac gctcgagatc cgctgaatag 301 agtcgacaca atcggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac 361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 421 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 481 gcctgaatgg cgaatggccc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 541 acaccgcata tagtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 601 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 661 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 721 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 1021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 1081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 1141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 1201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 1261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 1321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 1381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 1441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 1501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 1561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 1621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 1681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 1741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 1801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 1861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 2041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 2101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 2161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 2221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 2281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2641 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2701 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 2761 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 2821 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 2881 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 2941 aggaaacagc t //