LOCUS Exported 5151 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Provides alcR as GreenGate coding sequence module.. ACCESSION . VERSION . KEYWORDS pGGC011 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5151) AUTHORS Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. JOURNAL PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PUBMED 24376629 REFERENCE 2 (bases 1 to 5151) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5151 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(419..438) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1721..1740 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1974..1991 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 2212..2230 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 2212..2229 /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4737..4756) /label=T7 /note="T7 promoter, forward primer" promoter complement(4738..4756) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4766..4788) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(4982..5001) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5101..5123 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg 2221 acactataga agtgaagctt ggtctcaggc tccatggcag atacgcgccg acgccagaat 2281 catagctgcg atccctgtcg caagggcaag cgacgctgtg atgccccgga aaatagaaac 2341 gaggccaatg aaaacggctg ggtttcgtgt tcaaattgca agcgttggaa caaggattgt 2401 accttcaatt ggctctcatc ccaacgctcc aaggcaaaag gggctgcacc tagagcgaga 2461 acaaagaaag ccaggaccgc aacaaccacc agtgaaccat caacttcagc tgcaacaatc 2521 cctacaccgg aaagtgacaa tcacgatgcg cctccagtca taaactctca cgacgcgctc 2581 ccgagctgga ctcaggggct actctcccac cccggcgacc ttttcgattt cagccactct 2641 gctattcccg caaatgcaga agatgcggcc aacgtgcagt cagacgcacc ttttccgtgg 2701 gatctagcca tccccggtga tttcagcatg ggccaacagc tcgagaaacc tctcagtccg 2761 ctcagttttc aagcagtcct tcttccgccc catagcccga acacggatga cctcattcgc 2821 gagctggaag agcagactac agatccggac tcggttaccg atactaatag tgtacaacag 2881 gtcgctcaag atggatcgct atggtctgat cggcagtcgc cgctactgcc tgagaacagt 2941 ctgtgcatgg cctcagacag cacagcacgg cgatatgccc gttccacaat gacgaagaat 3001 ctgatgcgaa tctaccacga tagtatggag aatgcactgt cctgctggct gacagagcac 3061 aattgtccat actccgacca gatcagctac ctgccgccca agcagcgggc ggaatggggc 3121 ccgaactggt caaacaggat gtgcatccgg gtgtgccggc tagatcgcgt atctacctca 3181 ttacgcgggc gcgccctgag tgcggaagag gacaaagccg cagcccgagc cctgcatctg 3241 gcgatcgtag cttttgcgtc gcaatggacg cagcatgcgc agaggggggc tgggctaaat 3301 gttcctgcag acatagccgc cgatgagagg tccatccgga ggaacgcctg gaatgaagca 3361 cgccatgcct tgcagcacac gacagggatt ccatcattcc gggttatatt tgcgaatatc 3421 atcttttctc tcacgcagag tgtgctggat gatgatgagc agcacggtat gggtgcacgt 3481 ctagacaagc tactcgaaaa tgacggtgcg cccgtgttcc tggaaaccgc gaaccgtcag 3541 ctttatacat tccgacataa gtttgcacga atgcaacgcc gcggtaaggc tttcaacagg 3601 ctcccgggag gatctgtcgc atcgacattc gccggtattt tcgagacacc gacgccgtcg 3661 tctgaaagcc cacagcttga cccggttgtg gccagtgagg agcatcgcag tacattaagc 3721 cttatgttct ggctagggat catgttcgat acactaagcg ctgcaatgta ccagcgacca 3781 ctcgtggtgt cagatgagga tagccagata tcatcggcat ctccaccaag gcgcggcgct 3841 gaaacgccga tcaacctaga ctgctgggag cccccgagac aggtcccgag caatcaagaa 3901 aagagcgacg tatggggcga cctcttcctc cgcacctcgg actctctccc agatcacgaa 3961 tcccacacac aaatctctca gccagcggct cgatggccct gcacctacga acaggccgcc 4021 gccgctctct cctctgcaac gcccgtcaaa gtcctcctct accgccgcgt cacgcagctc 4081 caaaccctcc tctatcgcgg cgccagccct gcccgccttg aagcggccat ccagagaacg 4141 ctctacgttt ataatcactg gacagcgaag taccaaccat ttatgcagga ctgcgttgct 4201 aaccacgagc tcctcccttc gcgcatccag tcttggtacg tcattctaga cggtcactgg 4261 catctagccg cgatgttgct agcggacgtt ttggagagca tcgaccgcga ttcgtactct 4321 gatatcaacc acatcgacct tgtaacaaag ctaaggctcg ataatgcact agcagttagt 4381 gcccttgcgc gctcttcact ccgaggccag gagctggacc cgggcaaagc atctccgatg 4441 tatcgccatt tccatgattc tctgaccgag gtggcattcc tggtagaacc gtggaccgtc 4501 gttcttattc actcgtttgc caaagctgcg tatatcttgc tggactgttt agatctggac 4561 ggccaaggaa atgcactagc ggggtacctg cagctgcggc aaaattgcaa ctactgcatt 4621 cgggcgctgc aatttctggg caggaagtcg gatatggcgg cgctggttgc gaaggattta 4681 gagagaggtt tgaatgggaa agttgacagc tttttgtcag tgagaccgaa ttctcgccct 4741 atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 4801 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 4861 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga 4921 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 4981 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 5041 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 5101 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a //