LOCUS       Exported                1080 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  2nd gen lentivector expressing shRNA from H1 promoter and GFP. For 
            direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from 
            pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    pLVTHM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    65..182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      267..276
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             273..989
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             273..989
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(318..339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(579..598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     926..947
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catttcaggt gtcgtgagga atttcgacat ttaaatttaa ttaatctcga cggtatcggt
       61 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      121 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      181 ttatcgatca cgagactagc ctcgaggttt aaactacggg atcttcgaag gcctaagctt
      241 acgcgcgcgt cctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
      301 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      361 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      421 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      481 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      541 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      601 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      661 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      721 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      781 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      841 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      901 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      961 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct
     1021 agctagctag tcgagctcaa gcttcgggga ctagtcatat gataatcaac ctctggatta
//
